Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise

L A Dixon; A E Dobbins; H K Pulker; J M Butler; P M Vallone; M D Coble; W Parson; B Berger; P Grubwieser; Helle Smidt Mogensen; Niels Morling; Karsten Nielsen; Juan Jose Sanchez Sanchez; E Petkovski; A Carracedo; P Sanchez-Diz; E Ramos-Luis; M Brion; J A Irwin; R S Just; O Loreille; T J Parsons; D Syndercombe-Court; H Schmitter; B Stradmann-Bellinghausen; K Bender; P Gill

doi:10.1016/j.forsciint.2005.11.011

Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise

L A Dixon, A E Dobbins, H K Pulker, J M Butler, P M Vallone, M D Coble, W Parson, B Berger, P Grubwieser, Helle Smidt Mogensen, Niels Morling, Karsten Nielsen, Juan Jose Sanchez Sanchez, E Petkovski, A Carracedo, P Sanchez-Diz, E Ramos-Luis, M Brion, J A Irwin, R S JustO Loreille, T J Parsons, D Syndercombe-Court, H Schmitter, B Stradmann-Bellinghausen, K Bender, P Gill

Section of Forensic Genetics

108 Citations (Scopus)

Abstract

Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.

Original language	English
Journal	Forensic Science International
Volume	164
Issue number	1
Pages (from-to)	33-44
Number of pages	11
ISSN	0379-0738
DOIs	https://doi.org/10.1016/j.forsciint.2005.11.011
Publication status	Published - 2005

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10.1016/j.forsciint.2005.11.011

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Dixon, L. A., Dobbins, A. E., Pulker, H. K., Butler, J. M., Vallone, P. M., Coble, M. D., Parson, W., Berger, B., Grubwieser, P., Mogensen, H. S., Morling, N., Nielsen, K., Sanchez Sanchez, J. J., Petkovski, E., Carracedo, A., Sanchez-Diz, P., Ramos-Luis, E., Brion, M., Irwin, J. A., ... Gill, P. (2005). Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise. Forensic Science International, 164(1), 33-44. https://doi.org/10.1016/j.forsciint.2005.11.011

Dixon, LA, Dobbins, AE, Pulker, HK, Butler, JM, Vallone, PM, Coble, MD, Parson, W, Berger, B, Grubwieser, P, Mogensen, HS , Morling, N, Nielsen, K, Sanchez Sanchez, JJ, Petkovski, E, Carracedo, A, Sanchez-Diz, P, Ramos-Luis, E, Brion, M, Irwin, JA, Just, RS, Loreille, O, Parsons, TJ, Syndercombe-Court, D, Schmitter, H, Stradmann-Bellinghausen, B, Bender, K & Gill, P 2005, 'Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise', Forensic Science International, vol. 164, no. 1, pp. 33-44. https://doi.org/10.1016/j.forsciint.2005.11.011

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title = "Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise",

abstract = "Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.",

author = "Dixon, {L A} and Dobbins, {A E} and Pulker, {H K} and Butler, {J M} and Vallone, {P M} and Coble, {M D} and W Parson and B Berger and P Grubwieser and Mogensen, {Helle Smidt} and Niels Morling and Karsten Nielsen and {Sanchez Sanchez}, {Juan Jose} and E Petkovski and A Carracedo and P Sanchez-Diz and E Ramos-Luis and M Brion and Irwin, {J A} and Just, {R S} and O Loreille and Parsons, {T J} and D Syndercombe-Court and H Schmitter and B Stradmann-Bellinghausen and K Bender and P Gill",

note = "Keywords: Analysis of Variance; Blood; DNA Degradation, Necrotic; DNA Fingerprinting; Europe; Forensic Genetics; Genotype; Humans; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Saliva; Tandem Repeat Sequences",

year = "2005",

doi = "10.1016/j.forsciint.2005.11.011",

language = "English",

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T1 - Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise

AU - Dixon, L A

AU - Dobbins, A E

AU - Pulker, H K

AU - Butler, J M

AU - Vallone, P M

AU - Coble, M D

AU - Parson, W

AU - Berger, B

AU - Grubwieser, P

AU - Mogensen, Helle Smidt

AU - Morling, Niels

AU - Nielsen, Karsten

AU - Sanchez Sanchez, Juan Jose

AU - Petkovski, E

AU - Carracedo, A

AU - Sanchez-Diz, P

AU - Ramos-Luis, E

AU - Brion, M

AU - Irwin, J A

AU - Just, R S

AU - Loreille, O

AU - Parsons, T J

AU - Syndercombe-Court, D

AU - Schmitter, H

AU - Stradmann-Bellinghausen, B

AU - Bender, K

AU - Gill, P

N1 - Keywords: Analysis of Variance; Blood; DNA Degradation, Necrotic; DNA Fingerprinting; Europe; Forensic Genetics; Genotype; Humans; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Saliva; Tandem Repeat Sequences

PY - 2005

Y1 - 2005

N2 - Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.

AB - Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.

U2 - 10.1016/j.forsciint.2005.11.011

DO - 10.1016/j.forsciint.2005.11.011

M3 - Journal article

C2 - 16343834

SN - 0379-0738

VL - 164

SP - 33

EP - 44

JO - Forensic Science International

JF - Forensic Science International

IS - 1

ER -