An immune response study of oakmoss absolute and its constituents atranol and chloroatranol

Charlotte Menné Bonefeld; Morten Milek Nielsen; Elena Gimenéz-Arnau; Matthieu Lang; Marie Torp Vennegaard; Carsten Geisler; Jeanne Duus Johansen; Jean-Pierre Lepoittevin

doi:10.1111/cod.12177

An immune response study of oakmoss absolute and its constituents atranol and chloroatranol

Charlotte Menné Bonefeld, Morten Milek Nielsen, Elena Gimenéz-Arnau, Matthieu Lang, Marie Torp Vennegaard, Carsten Geisler, Jeanne Duus Johansen, Jean-Pierre Lepoittevin

11 Citations (Scopus)

Abstract

BACKGROUND: Atranol and chloroatranol are the main allergens of oakmoss absolute. However, the immune responses induced by these substances are poorly characterized.

OBJECTIVES: To characterize immune responses induced by atranol, chloroatranol and oakmoss absolute in mice.

METHODS: Mice were sensitized and challenged with various concentrations of atranol, chloroatranol, and oakmoss absolute. The immune responses were analysed as B cell infiltration, T cell proliferation in the draining lymph nodes, and expression of interleukin (IL)-18, IL-1β and tumour necrosis factor-α in skin. The cytotoxicity of atranol and chloroatranol against keratinocytes was determined.

RESULTS: Sensitization experiments showed that atranol, chloroatranol and oakmoss induced sensitization when applied in high concentrations. Challenge experiments showed that even low concentrations of atranol and chloroatranol induced sensitization. In parallel, atranol and chloroatranol elicited challenge reactions following sensitization with oakmoss. The magnitude of the immune response to the three allergens increased in the following order: atranol, chloroatranol, and oakmoss. The expression of proinflammatory cytokines was induced by chloroatranol and oakmoss, but not by atranol. Chloroatranol was found to be more cytotoxic than atranol against keratinocytes.

CONCLUSIONS: Atranol and chloroatranol can elicit both sensitization and challenge reactions, but the mixture of allergens in oakmoss absolute is more potent than atranol and chloroatranol alone.

Original language	English
Journal	Contact Dermatitis
Volume	70
Issue number	5
Pages (from-to)	282-290
Number of pages	9
ISSN	0105-1873
DOIs	https://doi.org/10.1111/cod.12177
Publication status	Published - May 2014

Keywords

Animals
Antigens, CD19
B-Lymphocytes
Benzaldehydes
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Cell Line
Cell Proliferation
Dermatitis, Allergic Contact
Interleukin-18
Interleukin-1beta
Keratinocytes
Mice
Mice, Inbred CBA
Patch Tests
Resins, Plant
Terpenes
Tumor Necrosis Factor-alpha

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10.1111/cod.12177

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@article{0103d30d62f44bb883c5d605c10ebbb5,

title = "An immune response study of oakmoss absolute and its constituents atranol and chloroatranol",

abstract = "BACKGROUND: Atranol and chloroatranol are the main allergens of oakmoss absolute. However, the immune responses induced by these substances are poorly characterized.OBJECTIVES: To characterize immune responses induced by atranol, chloroatranol and oakmoss absolute in mice.METHODS: Mice were sensitized and challenged with various concentrations of atranol, chloroatranol, and oakmoss absolute. The immune responses were analysed as B cell infiltration, T cell proliferation in the draining lymph nodes, and expression of interleukin (IL)-18, IL-1β and tumour necrosis factor-α in skin. The cytotoxicity of atranol and chloroatranol against keratinocytes was determined.RESULTS: Sensitization experiments showed that atranol, chloroatranol and oakmoss induced sensitization when applied in high concentrations. Challenge experiments showed that even low concentrations of atranol and chloroatranol induced sensitization. In parallel, atranol and chloroatranol elicited challenge reactions following sensitization with oakmoss. The magnitude of the immune response to the three allergens increased in the following order: atranol, chloroatranol, and oakmoss. The expression of proinflammatory cytokines was induced by chloroatranol and oakmoss, but not by atranol. Chloroatranol was found to be more cytotoxic than atranol against keratinocytes.CONCLUSIONS: Atranol and chloroatranol can elicit both sensitization and challenge reactions, but the mixture of allergens in oakmoss absolute is more potent than atranol and chloroatranol alone.",

keywords = "Animals, Antigens, CD19, B-Lymphocytes, Benzaldehydes, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Line, Cell Proliferation, Dermatitis, Allergic Contact, Interleukin-18, Interleukin-1beta, Keratinocytes, Mice, Mice, Inbred CBA, Patch Tests, Resins, Plant, Terpenes, Tumor Necrosis Factor-alpha",

author = "Bonefeld, {Charlotte Menn{\'e}} and Nielsen, {Morten Milek} and Elena Gimen{\'e}z-Arnau and Matthieu Lang and Vennegaard, {Marie Torp} and Carsten Geisler and Johansen, {Jeanne Duus} and Jean-Pierre Lepoittevin",

note = "{\textcopyright} 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",

year = "2014",

month = may,

doi = "10.1111/cod.12177",

language = "English",

volume = "70",

pages = "282--290",

journal = "Contact Dermatitis",

issn = "0105-1873",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - An immune response study of oakmoss absolute and its constituents atranol and chloroatranol

AU - Bonefeld, Charlotte Menné

AU - Nielsen, Morten Milek

AU - Gimenéz-Arnau, Elena

AU - Lang, Matthieu

AU - Vennegaard, Marie Torp

AU - Geisler, Carsten

AU - Johansen, Jeanne Duus

AU - Lepoittevin, Jean-Pierre

PY - 2014/5

Y1 - 2014/5

N2 - BACKGROUND: Atranol and chloroatranol are the main allergens of oakmoss absolute. However, the immune responses induced by these substances are poorly characterized.OBJECTIVES: To characterize immune responses induced by atranol, chloroatranol and oakmoss absolute in mice.METHODS: Mice were sensitized and challenged with various concentrations of atranol, chloroatranol, and oakmoss absolute. The immune responses were analysed as B cell infiltration, T cell proliferation in the draining lymph nodes, and expression of interleukin (IL)-18, IL-1β and tumour necrosis factor-α in skin. The cytotoxicity of atranol and chloroatranol against keratinocytes was determined.RESULTS: Sensitization experiments showed that atranol, chloroatranol and oakmoss induced sensitization when applied in high concentrations. Challenge experiments showed that even low concentrations of atranol and chloroatranol induced sensitization. In parallel, atranol and chloroatranol elicited challenge reactions following sensitization with oakmoss. The magnitude of the immune response to the three allergens increased in the following order: atranol, chloroatranol, and oakmoss. The expression of proinflammatory cytokines was induced by chloroatranol and oakmoss, but not by atranol. Chloroatranol was found to be more cytotoxic than atranol against keratinocytes.CONCLUSIONS: Atranol and chloroatranol can elicit both sensitization and challenge reactions, but the mixture of allergens in oakmoss absolute is more potent than atranol and chloroatranol alone.

AB - BACKGROUND: Atranol and chloroatranol are the main allergens of oakmoss absolute. However, the immune responses induced by these substances are poorly characterized.OBJECTIVES: To characterize immune responses induced by atranol, chloroatranol and oakmoss absolute in mice.METHODS: Mice were sensitized and challenged with various concentrations of atranol, chloroatranol, and oakmoss absolute. The immune responses were analysed as B cell infiltration, T cell proliferation in the draining lymph nodes, and expression of interleukin (IL)-18, IL-1β and tumour necrosis factor-α in skin. The cytotoxicity of atranol and chloroatranol against keratinocytes was determined.RESULTS: Sensitization experiments showed that atranol, chloroatranol and oakmoss induced sensitization when applied in high concentrations. Challenge experiments showed that even low concentrations of atranol and chloroatranol induced sensitization. In parallel, atranol and chloroatranol elicited challenge reactions following sensitization with oakmoss. The magnitude of the immune response to the three allergens increased in the following order: atranol, chloroatranol, and oakmoss. The expression of proinflammatory cytokines was induced by chloroatranol and oakmoss, but not by atranol. Chloroatranol was found to be more cytotoxic than atranol against keratinocytes.CONCLUSIONS: Atranol and chloroatranol can elicit both sensitization and challenge reactions, but the mixture of allergens in oakmoss absolute is more potent than atranol and chloroatranol alone.

KW - Animals

KW - Antigens, CD19

KW - B-Lymphocytes

KW - Benzaldehydes

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Cell Line

KW - Cell Proliferation

KW - Dermatitis, Allergic Contact

KW - Interleukin-18

KW - Interleukin-1beta

KW - Keratinocytes

KW - Mice

KW - Mice, Inbred CBA

KW - Patch Tests

KW - Resins, Plant

KW - Terpenes

KW - Tumor Necrosis Factor-alpha

U2 - 10.1111/cod.12177

DO - 10.1111/cod.12177

M3 - Journal article

C2 - 24460794

SN - 0105-1873

VL - 70

SP - 282

EP - 290

JO - Contact Dermatitis

JF - Contact Dermatitis

IS - 5

ER -

An immune response study of oakmoss absolute and its constituents atranol and chloroatranol

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Keywords

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