Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

Yousif Subhi; Marie Krogh Nielsen; Christopher Molbech; Torben Lykke Sørensen

doi:10.1111/ceo.13152

Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

Yousif Subhi, Marie Krogh Nielsen, Christopher Molbech, Torben Lykke Sørensen

Department of Clinical Medicine

15 Citations (Scopus)

Abstract

Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2⁺- and CX3CR1⁺-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2⁺ and CX3CR1⁺ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2⁺ and CX3CR1⁺ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

Original language	English
Journal	Clinical and Experimental Ophthalmology
Volume	46
Issue number	6
Pages (from-to)	661-669
ISSN	1442-6404
DOIs	https://doi.org/10.1111/ceo.13152
Publication status	Published - Aug 2018

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Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy. / Subhi, Yousif; Nielsen, Marie Krogh; Molbech, Christopher et al.
In: Clinical and Experimental Ophthalmology, Vol. 46, No. 6, 08.2018, p. 661-669.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy",

abstract = "Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.",

author = "Yousif Subhi and Nielsen, {Marie Krogh} and Christopher Molbech and S{\o}rensen, {Torben Lykke}",

year = "2018",

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T1 - Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

AU - Subhi, Yousif

AU - Nielsen, Marie Krogh

AU - Molbech, Christopher

AU - Sørensen, Torben Lykke

PY - 2018/8

Y1 - 2018/8

N2 - Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

AB - Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

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DO - 10.1111/ceo.13152

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SN - 1442-6404

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JO - Clinical and Experimental Ophthalmology

JF - Clinical and Experimental Ophthalmology

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ER -

Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

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