Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

Yousif Subhi; Marie Krogh Nielsen; Christopher Molbech; Torben Lykke Sørensen

doi:10.1111/ceo.13152

Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

Yousif Subhi, Marie Krogh Nielsen, Christopher Molbech, Torben Lykke Sørensen

Institut for Klinisk Medicin

15 Citationer (Scopus)

Abstract

Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2⁺- and CX3CR1⁺-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2⁺ and CX3CR1⁺ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2⁺ and CX3CR1⁺ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

Originalsprog	Engelsk
Tidsskrift	Clinical and Experimental Ophthalmology
Vol/bind	46
Udgave nummer	6
Sider (fra-til)	661-669
ISSN	1442-6404
DOI	https://doi.org/10.1111/ceo.13152
Status	Udgivet - aug. 2018

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10.1111/ceo.13152

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Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy. / Subhi, Yousif; Nielsen, Marie Krogh; Molbech, Christopher et al.

I: Clinical and Experimental Ophthalmology, Bind 46, Nr. 6, 08.2018, s. 661-669.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy",

abstract = "Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.",

author = "Yousif Subhi and Nielsen, {Marie Krogh} and Christopher Molbech and S{\o}rensen, {Torben Lykke}",

year = "2018",

month = aug,

doi = "10.1111/ceo.13152",

language = "English",

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pages = "661--669",

journal = "Clinical and Experimental Ophthalmology",

issn = "1442-6404",

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TY - JOUR

T1 - Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

AU - Subhi, Yousif

AU - Nielsen, Marie Krogh

AU - Molbech, Christopher

AU - Sørensen, Torben Lykke

PY - 2018/8

Y1 - 2018/8

N2 - Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

AB - Background: We investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV). Methods: We recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case–control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2+- and CX3CR1+-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR. Results: Patients with neovascular AMD had significantly increased proportion of CCR2+ and CX3CR1+ non-classical monocytes. PCV type 1 was associated with significantly increased CCR2+ and CX3CR1+ in all monocyte subsets when compared to PCV type 2. Conclusions: Neovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

U2 - 10.1111/ceo.13152

DO - 10.1111/ceo.13152

M3 - Journal article

C2 - 29360187

SN - 1442-6404

VL - 46

SP - 661

EP - 669

JO - Clinical and Experimental Ophthalmology

JF - Clinical and Experimental Ophthalmology

IS - 6

ER -

Altered proportion of CCR2+ and CX3CR1+ circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

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