Abstract
The photoreactions of 9-[6-(4-azidobenzamido)hexylamino]acridine (AHA) and 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) with double stranded DNA result in formation of single strand nicks and alkali labile sites (adducts) with an efficiency of 6 x 10(-3) nicks per AHA and 3 x 10(-2) nicks per DHA molecule. The alkali dependent DNA cleavage by AHA shows a pronounced A+G preference whereas that by DHA is practically sequence independent. In the presence of diacridines, however, DHA exhibits a preference for cleavage at guanosines. These DNA photocleaving reagents could be useful for DNA photofootprinting and photosequencing.
| Original language | English |
|---|---|
| Journal | Nucleic Acids Research |
| Volume | 16 |
| Issue number | 9 |
| Pages (from-to) | 3877-88 |
| Number of pages | 12 |
| ISSN | 0305-1048 |
| Publication status | Published - 11 May 1988 |
Keywords
- Adenosine
- Aminoacridines/pharmacology
- Azides/pharmacology
- Base Sequence
- DNA/drug effects
- DNA Restriction Enzymes
- Guanosine
- Kinetics
- Light
- Photolysis
- Plasmids
- Ultraviolet Rays