A standardized surgical technique for rat superior cervical ganglionectomy

Luis Emilio Savastano; Analía Elizabeth Castro; Marcos René Fitt; Martin Fredensborg Rath; Horacio Eduardo Romeo; Estela Maris Muñoz

doi:10.1016/j.jneumeth.2010.07.007

A standardized surgical technique for rat superior cervical ganglionectomy

Luis Emilio Savastano, Analía Elizabeth Castro, Marcos René Fitt, Martin Fredensborg Rath, Horacio Eduardo Romeo, Estela Maris Muñoz

Neuronal Signalling

39 Citations (Scopus)

Abstract

Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-step description of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and after surgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.

Original language	English
Journal	Journal of Neuroscience Methods
Volume	192
Issue number	1
Pages (from-to)	22-33
Number of pages	12
ISSN	0165-0270
DOIs	https://doi.org/10.1016/j.jneumeth.2010.07.007
Publication status	Published - Sept 2010

Keywords

Animals
Disease Models, Animal
Female
Ganglionectomy
Horner Syndrome
Male
Neck
Neurofilament Proteins
Rats
Rats, Wistar
Superior Cervical Ganglion

Access to Document

10.1016/j.jneumeth.2010.07.007

Cite this

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title = "A standardized surgical technique for rat superior cervical ganglionectomy",

abstract = "Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-step description of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and after surgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.",

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author = "Savastano, {Luis Emilio} and Castro, {Anal{\'i}a Elizabeth} and Fitt, {Marcos Ren{\'e}} and Rath, {Martin Fredensborg} and Romeo, {Horacio Eduardo} and Mu{\~n}oz, {Estela Maris}",

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AU - Savastano, Luis Emilio

AU - Castro, Analía Elizabeth

AU - Fitt, Marcos René

AU - Rath, Martin Fredensborg

AU - Romeo, Horacio Eduardo

AU - Muñoz, Estela Maris

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Y1 - 2010/9

N2 - Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-step description of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and after surgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.

AB - Superior cervical ganglionectomy (SCGx) is a valuable microsurgical model to study the role of the sympathetic nervous system in a vast array of physiological and pathological processes, including homeostatic regulation, circadian biology and the dynamics of neuronal dysfunction and recovery after injury. Despite having several experimental applications in the rat, a thorough description of a standardized procedure has never been published. Here, we provide a brief review of the principal features and experimental uses of the SCGx, the surgical anatomy of the neck and sympathetic cervical chain, and a step-by-step description of how to consistently remove the superior cervical ganglia through the omohyoid muscle or the carotid triangle. Furthermore, we suggest procedures and precautions to be taken during and after surgery to optimize results and describe tools to validate surgical success. We expect that the following standardized and optimized protocol will allow researchers to organize knowledge into a cohesive framework in those areas where the SCGx is applied.

KW - Animals

KW - Disease Models, Animal

KW - Female

KW - Ganglionectomy

KW - Horner Syndrome

KW - Male

KW - Neck

KW - Neurofilament Proteins

KW - Rats

KW - Rats, Wistar

KW - Superior Cervical Ganglion

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