A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation

Lejla Gitte Ravn-Haren; Susanne Gjedsted Bügel; Britta Naimi Krath; Tien Hoac; Jan Stagsted; Karina Jørgensen; June R. Bresson; Erik H. Larsen; Lars Ove Dragsted

doi:10.1017/S0007114507825153

A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation

Lejla Gitte Ravn-Haren, Susanne Gjedsted Bügel, Britta Naimi Krath, Tien Hoac, Jan Stagsted, Karina Jørgensen, June R. Bresson, Erik H. Larsen, Lars Ove Dragsted

56 Citations (Scopus)

Abstract

Increased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 £ 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 mg/d for selenate and Se-enriched yeast, and about 480 mg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.

Original language	English
Journal	British Journal of Nutrition
Volume	99
Issue number	4
Pages (from-to)	883-892
Number of pages	10
ISSN	0007-1145
DOIs	https://doi.org/10.1017/S0007114507825153
Publication status	Published - 2007

Keywords

Former LIFE faculty
Selenium
Oxidative defence
Electrophile response elements
Gluththione peroxidase
blood lipids

Access to Document

10.1017/S0007114507825153

Cite this

Ravn-Haren, L. G., Bügel, S. G., Krath, B. N., Hoac, T., Stagsted, J., Jørgensen, K., Bresson, J. R., Larsen, E. H., & Dragsted, L. O. (2007). A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation. British Journal of Nutrition, 99(4), 883-892. https://doi.org/10.1017/S0007114507825153

A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk : effects on oxidative defence regulation. / Ravn-Haren, Lejla Gitte; Bügel, Susanne Gjedsted; Krath, Britta Naimi et al.

In: British Journal of Nutrition, Vol. 99, No. 4, 2007, p. 883-892.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk: effects on oxidative defence regulation",

abstract = "Increased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 £ 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 mg/d for selenate and Se-enriched yeast, and about 480 mg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation. ",

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author = "Ravn-Haren, {Lejla Gitte} and B{\"u}gel, {Susanne Gjedsted} and Krath, {Britta Naimi} and Tien Hoac and Jan Stagsted and Karina J{\o}rgensen and Bresson, {June R.} and Larsen, {Erik H.} and Dragsted, {Lars Ove}",

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T1 - A short-term intervention trial with selenate, selenium-enriched yeast and selenium-enriched milk

T2 - effects on oxidative defence regulation

AU - Ravn-Haren, Lejla Gitte

AU - Bügel, Susanne Gjedsted

AU - Krath, Britta Naimi

AU - Hoac, Tien

AU - Stagsted, Jan

AU - Jørgensen, Karina

AU - Bresson, June R.

AU - Larsen, Erik H.

AU - Dragsted, Lars Ove

PY - 2007

Y1 - 2007

N2 - Increased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 £ 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 mg/d for selenate and Se-enriched yeast, and about 480 mg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.

AB - Increased Se intakes have been associated with decreased risk of cancer and CVD. Several mechanisms have been proposed, including antioxidant effects through selenoproteins, induction of carcinogen metabolism and effects on the blood lipid profile. In a 4 £ 1 week randomised, double-blind cross-over study, healthy young men supplemented their usual diet with selenate, Se-enriched yeast, Se-enriched milk or placebo (Se dose was 300 mg/d for selenate and Se-enriched yeast, and about 480 mg/d for Se-enriched milk) followed by 8-week washout periods. All Se sources increased serum Se levels after supplementation for 1 week. The effect of the organic forms did not differ significantly and both increased serum Se more than selenate. Conversely, thrombocyte glutathione peroxidase (GPX) was increased in the periods where subjects were supplemented with selenate but not in those where they were given Se-enriched yeast or Se-enriched milk. We found no effect on plasma lipid resistance to oxidation, total cholesterol, TAG, HDL- and LDL-cholesterol, GPX, glutathione reductase (GR) and glutathione S-transferase (GST) activities measured in erythrocytes, GPX and GR activities determined in plasma, or GR and GST activities in thrombocytes. Leucocyte expression of genes encoding selenoproteins (GPX1, TrR1 and SelP), and of electrophile response element-regulated genes (GCLC, Fra1 and NQO1) were likewise unaffected at all time points following intervention. We conclude that thrombocyte GPX is specifically increased by short-term selenate supplementation, but not by short-term supplementation with organic Se. Short-term Se supplementation does not seem to affect blood lipid markers or expression and activity of selected enzymes and a transcription factor involved in glutathione-mediated detoxification and antioxidation.

KW - Former LIFE faculty

KW - Selenium

KW - Oxidative defence

KW - Electrophile response elements

KW - Gluththione peroxidase

KW - blood lipids

U2 - 10.1017/S0007114507825153

DO - 10.1017/S0007114507825153

M3 - Journal article

C2 - 17888202

SN - 0007-1145

VL - 99

SP - 883

EP - 892

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 4

ER -