TY - JOUR
T1 - A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens.
AU - Sundelin, Thomas
AU - Strømeng, Gunn Mari
AU - Gjærum, Halvor B.
AU - Amby, Daniel Buchvaldt
AU - Ørstad, Kari
AU - Jensen, Birgit
AU - Lund, Ole Søgaard
AU - Stensvand, Arne
PY - 2015/8/10
Y1 - 2015/8/10
N2 - Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.
AB - Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.
U2 - 10.1093/femsle/fnv130
DO - 10.1093/femsle/fnv130
M3 - Tidsskriftartikel
C2 - 26296707
SN - 0378-1097
VL - 362
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
M1 - fnv130
ER -