A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens.

Thomas Sundelin; Gunn Mari Strømeng; Halvor B. Gjærum; Daniel Buchvaldt Amby; Kari Ørstad; Birgit Jensen; Ole Søgaard Lund; Arne Stensvand

doi:10.1093/femsle/fnv130

A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens.

Thomas Sundelin, Gunn Mari Strømeng, Halvor B. Gjærum, Daniel Buchvaldt Amby, Kari Ørstad, Birgit Jensen, Ole Søgaard Lund, Arne Stensvand

5 Citationer (Scopus)

Abstract

Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.

Originalsprog	Dansk
Artikelnummer	fnv130
Tidsskrift	F E M S Microbiology Letters
Vol/bind	362
Antal sider	7
ISSN	0378-1097
DOI	https://doi.org/10.1093/femsle/fnv130
Status	Udgivet - 10 aug. 2015

Adgang til dokumentet

10.1093/femsle/fnv130

fnv130.pdfForlagets udgivne version, 528 KB

Citationsformater

@article{047f5b794fba4db28322aeb9cb7461b6,

title = "A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens.",

abstract = "Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.",

author = "Thomas Sundelin and Str{\o}meng, {Gunn Mari} and Gj{\ae}rum, {Halvor B.} and Amby, {Daniel Buchvaldt} and Kari {\O}rstad and Birgit Jensen and Lund, {Ole S{\o}gaard} and Arne Stensvand",

year = "2015",

month = aug,

day = "10",

doi = "10.1093/femsle/fnv130",

language = "Dansk",

volume = "362",

journal = "F E M S Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - A revision of the history of the Colletotrichum acutatum species complex in the Nordic countries based on herbarium specimens.

AU - Sundelin, Thomas

AU - Strømeng, Gunn Mari

AU - Gjærum, Halvor B.

AU - Amby, Daniel Buchvaldt

AU - Ørstad, Kari

AU - Jensen, Birgit

AU - Lund, Ole Søgaard

AU - Stensvand, Arne

PY - 2015/8/10

Y1 - 2015/8/10

N2 - Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.

AB - Herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. Species belonging to the Colletotrichum acutatum complex infect a range of important crops. As members of the C. acutatum complex are easily confused with other Colletotrichum species, molecular methods are central for the correct identification. We performed molecular analyses on 21 herbaria specimens, displaying anthracnose symptoms, collected in Norway and Denmark before the first confirmed findings of C. acutatum complex members in this region. Sequencing parts of the fungal ITS regions showed that members of the species complex were present in 13 of the 21 specimens collected in different parts of Norway and Denmark between 1948 and 1991, representing seven plant hosts (three cherry species, apple, raspberry and rhododendron). This is the first time herbarium specimens have been used to study these pathogens under Nordic conditions. Differences in the ITS sequences suggest the presence of different genotypes within the complex, indicating a well-established population.

U2 - 10.1093/femsle/fnv130

DO - 10.1093/femsle/fnv130

M3 - Tidsskriftartikel

C2 - 26296707

SN - 0378-1097

VL - 362

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

M1 - fnv130

ER -