A recombinase-mediated transcriptional induction system in transgenic plants.

T Hoff; K M Schnorr; J Mundy

A recombinase-mediated transcriptional induction system in transgenic plants.

T Hoff, K M Schnorr, J Mundy

83 Citations (Scopus)

Abstract

We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.

Original language	English
Journal	Plant Molecular Biology
Volume	45
Issue number	1
Pages (from-to)	41-9
Number of pages	8
ISSN	0167-4412
Publication status	Published - 2001

Cite this

@article{4cb7daa093a411dd86a6000ea68e967b,

title = "A recombinase-mediated transcriptional induction system in transgenic plants.",

abstract = "We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.",

author = "T Hoff and Schnorr, {K M} and J Mundy",

note = "Keywords: Acetyltransferases; Arabidopsis; Blotting, Southern; DNA, Plant; Gene Expression Regulation, Plant; Genetic Vectors; Glucuronidase; Heat; Integrases; Plants, Genetically Modified; Plasmids; Polymerase Chain Reaction; RNA, Plant; Recombinant Fusion Proteins; Trans-Activation (Genetics); Viral Proteins",

year = "2001",

language = "English",

volume = "45",

pages = "41--9",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - A recombinase-mediated transcriptional induction system in transgenic plants.

AU - Hoff, T

AU - Schnorr, K M

AU - Mundy, J

N1 - Keywords: Acetyltransferases; Arabidopsis; Blotting, Southern; DNA, Plant; Gene Expression Regulation, Plant; Genetic Vectors; Glucuronidase; Heat; Integrases; Plants, Genetically Modified; Plasmids; Polymerase Chain Reaction; RNA, Plant; Recombinant Fusion Proteins; Trans-Activation (Genetics); Viral Proteins

PY - 2001

Y1 - 2001

N2 - We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.

AB - We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.

M3 - Journal article

C2 - 11247605

SN - 0167-4412

VL - 45

SP - 41

EP - 49

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 1

ER -

A recombinase-mediated transcriptional induction system in transgenic plants.

Abstract

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