TY - JOUR
T1 - A recombinase-mediated transcriptional induction system in transgenic plants.
AU - Hoff, T
AU - Schnorr, K M
AU - Mundy, J
N1 - Keywords: Acetyltransferases; Arabidopsis; Blotting, Southern; DNA, Plant; Gene Expression Regulation, Plant; Genetic Vectors; Glucuronidase; Heat; Integrases; Plants, Genetically Modified; Plasmids; Polymerase Chain Reaction; RNA, Plant; Recombinant Fusion Proteins; Trans-Activation (Genetics); Viral Proteins
PY - 2001
Y1 - 2001
N2 - We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.
AB - We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.
M3 - Journal article
C2 - 11247605
SN - 0167-4412
VL - 45
SP - 41
EP - 49
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 1
ER -