A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells

P S Andersen; A Stryhn; B E Hansen; L Fugger; J Engberg; S Buus

A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells

P S Andersen, A Stryhn, B E Hansen, L Fugger, J Engberg, S Buus

106 Citations (Scopus)

Abstract

Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.

Original language	English
Journal	Proceedings of the National Academy of Science of the United States of America
Volume	93
Issue number	5
Pages (from-to)	1820-4
Number of pages	4
ISSN	0027-8424
Publication status	Published - 1996

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A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells. / Andersen, P S; Stryhn, A; Hansen, B E et al.

In: Proceedings of the National Academy of Science of the United States of America, Vol. 93, No. 5, 1996, p. 1820-4.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.",

author = "Andersen, {P S} and A Stryhn and Hansen, {B E} and L Fugger and J Engberg and S Buus",

note = "Keywords: Amino Acid Sequence; Animals; Antibody Specificity; Bacteriophages; Gene Library; Major Histocompatibility Complex; Mice; Mice, Inbred AKR; Molecular Sequence Data; Peptides; Recombinant Proteins; T-Lymphocytes",

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T1 - A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells

AU - Andersen, P S

AU - Stryhn, A

AU - Hansen, B E

AU - Fugger, L

AU - Engberg, J

AU - Buus, S

N1 - Keywords: Amino Acid Sequence; Animals; Antibody Specificity; Bacteriophages; Gene Library; Major Histocompatibility Complex; Mice; Mice, Inbred AKR; Molecular Sequence Data; Peptides; Recombinant Proteins; T-Lymphocytes

PY - 1996

Y1 - 1996

N2 - Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.

AB - Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.

M3 - Journal article

C2 - 8700842

SN - 0027-8424

VL - 93

SP - 1820

EP - 1824

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells

Abstract

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