A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Morgann C Reilly; Steven B Levery; Sherry A Castle; J Stacey Klutts; Tamara L Doering

doi:10.1074/jbc.M109.056226

A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Morgann C Reilly, Steven B Levery, Sherry A Castle, J Stacey Klutts, Tamara L Doering

Glycomics Program

14 Citations (Scopus)

Abstract

Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.

Original language	English
Journal	Journal of Biological Chemistry
Volume	284
Issue number	52
Pages (from-to)	36118-27
Number of pages	9
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.M109.056226
Publication status	Published - 2009

Access to Document

10.1074/jbc.M109.056226

Cite this

@article{70d2d440fc4d11de825d000ea68e967b,

title = "A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans",

abstract = "Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.",

author = "Reilly, {Morgann C} and Levery, {Steven B} and Castle, {Sherry A} and Klutts, {J Stacey} and Doering, {Tamara L}",

year = "2009",

doi = "10.1074/jbc.M109.056226",

language = "English",

volume = "284",

pages = "36118--27",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

number = "52",

}

TY - JOUR

T1 - A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

AU - Reilly, Morgann C

AU - Levery, Steven B

AU - Castle, Sherry A

AU - Klutts, J Stacey

AU - Doering, Tamara L

PY - 2009

Y1 - 2009

N2 - Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.

AB - Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.

U2 - 10.1074/jbc.M109.056226

DO - 10.1074/jbc.M109.056226

M3 - Journal article

C2 - 19864415

SN - 0021-9258

VL - 284

SP - 36118

EP - 36127

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 52

ER -

A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Abstract

Access to Document

Fingerprint

Cite this