A new RNA branching activity: the GIR1 ribozyme

TY - JOUR

T1 - A new RNA branching activity: the GIR1 ribozyme

AU - Nielsen, Henrik

AU - Johansen, Steinar D

N1 - Keywords: Catalysis; DNA, Catalytic; Nucleic Acid Conformation; RNA; RNA Caps; RNA, Catalytic

PY - 2006

Y1 - 2006

N2 - The formation of lariat intermediates during the first step of splicing of group II introns and spliceosomal introns is a well-studied fundamental reaction in molecular biology. Apart from this prominent example, there are surprisingly few occurrences of branched nucleotides or even 2',5'-phosphodiester bonds in biology. We recently described a new ribozyme, the GIR1 branching ribozyme, which catalyzes the formation of a tiny lariat that caps an mRNA. This new example together with work on artificial branching ribozymes and deoxyribozymes shows that branching is facile and points to the possibility that branching reactions could be more prevalent than previously recognized.

AB - The formation of lariat intermediates during the first step of splicing of group II introns and spliceosomal introns is a well-studied fundamental reaction in molecular biology. Apart from this prominent example, there are surprisingly few occurrences of branched nucleotides or even 2',5'-phosphodiester bonds in biology. We recently described a new ribozyme, the GIR1 branching ribozyme, which catalyzes the formation of a tiny lariat that caps an mRNA. This new example together with work on artificial branching ribozymes and deoxyribozymes shows that branching is facile and points to the possibility that branching reactions could be more prevalent than previously recognized.

U2 - 10.1016/j.bcmd.2006.11.001

DO - 10.1016/j.bcmd.2006.11.001

M3 - Journal article

C2 - 17188534

SN - 1079-9796

VL - 38

SP - 102

EP - 109

JO - Blood Cells, Molecules and Diseases

JF - Blood Cells, Molecules and Diseases

IS - 2

ER -