Abstract
We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N,N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20 % and 40 % excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMA-MAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins. [Figure not available: see fulltext.]
Original language | English |
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Journal | Microchimica Acta |
Volume | 183 |
Issue number | 7 |
Pages (from-to) | 2111-2121 |
Number of pages | 11 |
ISSN | 0026-3672 |
DOIs | |
Publication status | Published - 1 Jul 2016 |
Keywords
- 3-(Methacryloyl-oxy)propyl-trimethoxysilane
- 3-Trimethylsilanyl-prop-2-yne methacrylate
- Electro-osmotic flow
- N,N-Dimethylacrylamide
- Protein adsorption
- Protein separation
- Surface hydrophilicity
- Thiol-ene polymers