A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

TY - JOUR

T1 - A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

AU - Mesbah, Kiarach

AU - Mai, Thanh Duc

AU - Jensen, Thomas Glasdam

AU - Sola, Laura

AU - Chiari, Marcella

AU - Kutter, Jörg P.

AU - Taverna, Myriam

PY - 2016/7/1

Y1 - 2016/7/1

N2 - We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N,N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20 % and 40 % excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMA-MAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins. [Figure not available: see fulltext.]

AB - We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N,N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20 % and 40 % excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMA-MAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins. [Figure not available: see fulltext.]

KW - 3-(Methacryloyl-oxy)propyl-trimethoxysilane

KW - 3-Trimethylsilanyl-prop-2-yne methacrylate

KW - Electro-osmotic flow

KW - N,N-Dimethylacrylamide

KW - Protein adsorption

KW - Protein separation

KW - Surface hydrophilicity

KW - Thiol-ene polymers

UR - http://www.scopus.com/inward/record.url?scp=84964407550&partnerID=8YFLogxK

U2 - 10.1007/s00604-016-1825-4

DO - 10.1007/s00604-016-1825-4

M3 - Journal article

AN - SCOPUS:84964407550

SN - 0026-3672

VL - 183

SP - 2111

EP - 2121

JO - Microchimica Acta

JF - Microchimica Acta

IS - 7

ER -