Abstract
This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.
| Original language | English |
|---|---|
| Journal | International Journal of Pharmaceutics |
| Volume | 241 |
| Issue number | 2 |
| Pages (from-to) | 385-9 |
| Number of pages | 5 |
| ISSN | 0378-5173 |
| Publication status | Published - 25 Jul 2002 |
| Externally published | Yes |
Keywords
- Adjuvants, Immunologic
- Drug Delivery Systems
- Fluorescein-5-isothiocyanate
- Fluorescent Dyes
- Ovalbumin
- Saponins