A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

Stephanie Könnings; Melissa J Copland; Nigel M Davies; Thomas Rades

A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

Stephanie Könnings, Melissa J Copland, Nigel M Davies, Thomas Rades

31 Citationer (Scopus)

Abstract

This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.

Originalsprog	Engelsk
Tidsskrift	International Journal of Pharmaceutics
Vol/bind	241
Udgave nummer	2
Sider (fra-til)	385-9
Antal sider	5
ISSN	0378-5173
Status	Udgivet - 25 jul. 2002
Udgivet eksternt	Ja

Citationsformater

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title = "A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method",

abstract = "This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.",

keywords = "Adjuvants, Immunologic, Drug Delivery Systems, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Ovalbumin, Saponins",

author = "Stephanie K{\"o}nnings and Copland, {Melissa J} and Davies, {Nigel M} and Thomas Rades",

year = "2002",

month = jul,

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language = "English",

volume = "241",

pages = "385--9",

journal = "International Journal of Pharmaceutics",

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TY - JOUR

T1 - A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

AU - Könnings, Stephanie

AU - Copland, Melissa J

AU - Davies, Nigel M

AU - Rades, Thomas

PY - 2002/7/25

Y1 - 2002/7/25

N2 - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.

AB - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.

KW - Adjuvants, Immunologic

KW - Drug Delivery Systems

KW - Fluorescein-5-isothiocyanate

KW - Fluorescent Dyes

KW - Ovalbumin

KW - Saponins

M3 - Journal article

C2 - 12100866

SN - 0378-5173

VL - 241

SP - 385

EP - 389

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 2

ER -

A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

Abstract

Fingeraftryk

Citationsformater