A high-resolution comparative map between pig chromosome 17 and human chromosomes 4, 8, and 20: Identification of synteny breakpoints

TY - JOUR

T1 - A high-resolution comparative map between pig chromosome 17 and human chromosomes 4, 8, and 20: Identification of synteny breakpoints

AU - Lahbib-Mansais, Yvette

AU - Karlskov-Mortensen, Peter

AU - Mompart, Florence

AU - Milan, Denis

AU - Jørgensen, Claus Bøttcher

AU - Cirera, Susanna

AU - Gorodkin, Jan

AU - Faraut, Thomas

AU - Yerle, Martine

AU - Fredholm, Merete

PY - 2005

Y1 - 2005

N2 - We report on the construction of a high-resolution comparative map of porcine chromosome 17 (SSC17) focusing on evolutionary breakpoints with human chromosomes. The comparative map shows high homology with human chromosome 20 but suggests more limited homologies with other human chromosomes. SSC17 is of particular interest in studies of chromosomal organization due to the presence of QTLs that affect meat quality and carcass composition. A total of 158 pig ESTs available in databases or developed by the Sino-Danish Pig Genome Sequencing Consortium were mapped using the INRA-University of Minnesota porcine radiation hydrid panel. The high-resolution map was further anchored by fluorescence in situ hybridization. This study confirmed the extensive conservation between SSC17 and HSA20 and enabled the gene order to be determined. The homology of the SSC17 pericentromeric region was extended to other human chromosomes (HSA4, HSA8) and the chromosomal breakpoint boundaries were accurately defined. In total 15 breakpoints were identified.

AB - We report on the construction of a high-resolution comparative map of porcine chromosome 17 (SSC17) focusing on evolutionary breakpoints with human chromosomes. The comparative map shows high homology with human chromosome 20 but suggests more limited homologies with other human chromosomes. SSC17 is of particular interest in studies of chromosomal organization due to the presence of QTLs that affect meat quality and carcass composition. A total of 158 pig ESTs available in databases or developed by the Sino-Danish Pig Genome Sequencing Consortium were mapped using the INRA-University of Minnesota porcine radiation hydrid panel. The high-resolution map was further anchored by fluorescence in situ hybridization. This study confirmed the extensive conservation between SSC17 and HSA20 and enabled the gene order to be determined. The homology of the SSC17 pericentromeric region was extended to other human chromosomes (HSA4, HSA8) and the chromosomal breakpoint boundaries were accurately defined. In total 15 breakpoints were identified.

KW - Former LIFE faculty

KW - Pig

KW - SSC17

KW - RH map

KW - FISH

KW - Comparative mapping

KW - Human

KW - Synteny breakpoint

U2 - 10.1016/j.ygeno.2005.07.002

DO - 10.1016/j.ygeno.2005.07.002

M3 - Journal article

C2 - 16111857

SN - 0888-7543

VL - 86

SP - 405

EP - 413

JO - Genomics

JF - Genomics

IS - 4

ER -