TY - JOUR
T1 - A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood
AU - Hansen, Sanni
AU - Baptiste, Keith Edward
AU - Fjeldborg, Julie
AU - Betancourt, A.
AU - Horohov, D. W.
PY - 2014/4/15
Y1 - 2014/4/15
N2 - An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.
AB - An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described.The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of pro-inflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology.Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1β, IL-6, IL-8, TNF-α and TLR-4 were measured using qPCR.A significant age-related decrease for IL-1β and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found.In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as 'inflamm-aging'.
U2 - 10.1016/j.vetimm.2014.02.001
DO - 10.1016/j.vetimm.2014.02.001
M3 - Journal article
C2 - 24603016
SN - 0165-2427
VL - 158
SP - 238
EP - 243
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3-4
ER -