A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies

Nadejda Nikolajevna Larsen; Kim F. Michaelsen; Anders Pærregaard; Finn Kvist Vogensen; Mogens Jakobsen

doi:10.1080/08910600902907632

A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies

Nadejda Nikolajevna Larsen, Kim F. Michaelsen, Anders Pærregaard, Finn Kvist Vogensen, Mogens Jakobsen

11 Citations (Scopus)

Abstract

Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.

Original language	English
Journal	Microbiological Ecology in Health and Disease
Volume	21
Issue number	2
Pages (from-to)	95-99
Number of pages	5
ISSN	0891-060X
DOIs	https://doi.org/10.1080/08910600902907632
Publication status	Published - 2009

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A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies. / Larsen, Nadejda Nikolajevna ; Michaelsen, Kim F.; Pærregaard, Anders et al.
In: Microbiological Ecology in Health and Disease, Vol. 21, No. 2, 2009, p. 95-99.

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title = "A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies",

abstract = "Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.",

author = "Larsen, {Nadejda Nikolajevna} and Michaelsen, {Kim F.} and Anders P{\ae}rregaard and Vogensen, {Finn Kvist} and Mogens Jakobsen",

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T1 - A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies

AU - Larsen, Nadejda Nikolajevna

AU - Michaelsen, Kim F.

AU - Pærregaard, Anders

AU - Vogensen, Finn Kvist

AU - Jakobsen, Mogens

PY - 2009

Y1 - 2009

N2 - Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.

AB - Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.

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DO - 10.1080/08910600902907632

M3 - Journal article

SN - 0891-060X

VL - 21

SP - 95

EP - 99

JO - Microbiological Ecology in Health and Disease

JF - Microbiological Ecology in Health and Disease

IS - 2

ER -

A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies

Abstract

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