A chromogenic assay for limit dextrinase and pullulanase activity

Marie  Bøjstrup; Caspar Elo Christensen; Michael Skovbo Windahl; Anette Henriksen; Ole Hindsgaul

doi:10.1016/j.ab.2013.12.004

A chromogenic assay for limit dextrinase and pullulanase activity

Marie Bøjstrup, Caspar Elo Christensen, Michael Skovbo Windahl, Anette Henriksen, Ole Hindsgaul

6 Citations (Scopus)

Abstract

A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

Original language	English
Journal	Analytical Biochemistry
Volume	449
Pages (from-to)	45-51
Number of pages	7
ISSN	0003-2697
DOIs	https://doi.org/10.1016/j.ab.2013.12.004
Publication status	Published - 15 Mar 2014
Externally published	Yes

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title = "A chromogenic assay for limit dextrinase and pullulanase activity",

abstract = "A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.",

author = "Marie B{\o}jstrup and Christensen, {Caspar Elo} and Windahl, {Michael Skovbo} and Anette Henriksen and Ole Hindsgaul",

year = "2014",

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doi = "10.1016/j.ab.2013.12.004",

language = "English",

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journal = "Analytical Biochemistry",

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TY - JOUR

T1 - A chromogenic assay for limit dextrinase and pullulanase activity

AU - Bøjstrup, Marie

AU - Christensen, Caspar Elo

AU - Windahl, Michael Skovbo

AU - Henriksen, Anette

AU - Hindsgaul, Ole

PY - 2014/3/15

Y1 - 2014/3/15

N2 - A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

AB - A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

U2 - 10.1016/j.ab.2013.12.004

DO - 10.1016/j.ab.2013.12.004

M3 - Journal article

C2 - 24333247

SN - 0003-2697

VL - 449

SP - 45

EP - 51

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

A chromogenic assay for limit dextrinase and pullulanase activity

Abstract

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