A chromogenic assay for limit dextrinase and pullulanase activity

TY - JOUR

T1 - A chromogenic assay for limit dextrinase and pullulanase activity

AU - Bøjstrup, Marie

AU - Christensen, Caspar Elo

AU - Windahl, Michael Skovbo

AU - Henriksen, Anette

AU - Hindsgaul, Ole

PY - 2014/3/15

Y1 - 2014/3/15

N2 - A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

AB - A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl- maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

U2 - 10.1016/j.ab.2013.12.004

DO - 10.1016/j.ab.2013.12.004

M3 - Journal article

C2 - 24333247

SN - 0003-2697

VL - 449

SP - 45

EP - 51

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -