A cDNA clone for protein Z, a major barley endosperm albumin

Søren K. Rasmussen; H. Esteban Hopp; Anders Brandt; I. B. Svendsen; Jørn Hejgaard

doi:10.1007/BF02907781

A cDNA clone for protein Z, a major barley endosperm albumin

Søren K. Rasmussen^*, H. Esteban Hopp, Anders Brandt, I. B. Svendsen, Jørn Hejgaard

^*Corresponding author for this work

14 Citations (Scopus)

Abstract

An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

Original language	English
Journal	Carlsberg Research Communications
Volume	49
Issue number	3
Pages (from-to)	385-390
Number of pages	6
ISSN	0105-1938
DOIs	https://doi.org/10.1007/BF02907781
Publication status	Published - 1 Jan 1984

Keywords

gene family
in vitro protein synthesis
Lysine-rich storage protein
mRNA
N-terminal amino acid sequence
nucleotide sequence

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10.1007/BF02907781

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abstract = "An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.",

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T1 - A cDNA clone for protein Z, a major barley endosperm albumin

AU - Rasmussen, Søren K.

AU - Hopp, H. Esteban

AU - Brandt, Anders

AU - Svendsen, I. B.

AU - Hejgaard, Jørn

PY - 1984/1/1

Y1 - 1984/1/1

N2 - An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

AB - An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

KW - gene family

KW - in vitro protein synthesis

KW - Lysine-rich storage protein

KW - mRNA

KW - N-terminal amino acid sequence

KW - nucleotide sequence

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DO - 10.1007/BF02907781

M3 - Journal article

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JO - Carlsberg Research Communications

JF - Carlsberg Research Communications

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ER -

A cDNA clone for protein Z, a major barley endosperm albumin

Abstract

Keywords

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