A cDNA clone for protein Z, a major barley endosperm albumin

Søren K. Rasmussen; H. Esteban Hopp; Anders Brandt; I. B. Svendsen; Jørn Hejgaard

doi:10.1007/BF02907781

A cDNA clone for protein Z, a major barley endosperm albumin

Søren K. Rasmussen^*, H. Esteban Hopp, Anders Brandt, I. B. Svendsen, Jørn Hejgaard

^*Corresponding author af dette arbejde

14 Citationer (Scopus)

Abstract

An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

Originalsprog	Engelsk
Tidsskrift	Carlsberg Research Communications
Vol/bind	49
Udgave nummer	3
Sider (fra-til)	385-390
Antal sider	6
ISSN	0105-1938
DOI	https://doi.org/10.1007/BF02907781
Status	Udgivet - 1 jan. 1984

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title = "A cDNA clone for protein Z, a major barley endosperm albumin",

abstract = "An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.",

keywords = "gene family, in vitro protein synthesis, Lysine-rich storage protein, mRNA, N-terminal amino acid sequence, nucleotide sequence",

author = "Rasmussen, {S{\o}ren K.} and Hopp, {H. Esteban} and Anders Brandt and Svendsen, {I. B.} and J{\o}rn Hejgaard",

year = "1984",

month = jan,

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doi = "10.1007/BF02907781",

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T1 - A cDNA clone for protein Z, a major barley endosperm albumin

AU - Rasmussen, Søren K.

AU - Hopp, H. Esteban

AU - Brandt, Anders

AU - Svendsen, I. B.

AU - Hejgaard, Jørn

PY - 1984/1/1

Y1 - 1984/1/1

N2 - An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

AB - An 18 residue amino terminal sequence of the barley endosperm protein Z polypeptide is presented. In vitro translation of barley endosperm mRNA and subsequent immunoaffinity isolation using protein Z antibodies identified two major protein Z precursor polypeptides of M.W.44,000 and 46,000 among the translation products. Partial nucleotide sequence analysis identified a cDNA clone to encode the amino terminal portion of protein Z. Messenger RNA sequences 1,800 nucleotides in length, sufficient to code for the protein Z precursor polypeptides, were detected by hybridization to the protein Z cDNA probe. Hybridization of the probe to restriction endonuclease fragments of barley nuclear DNA suggests, that protein Z is encoded by a small multigene family.

KW - gene family

KW - in vitro protein synthesis

KW - Lysine-rich storage protein

KW - mRNA

KW - N-terminal amino acid sequence

KW - nucleotide sequence

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U2 - 10.1007/BF02907781

DO - 10.1007/BF02907781

M3 - Journal article

AN - SCOPUS:0000986785

SN - 0105-1938

VL - 49

SP - 385

EP - 390

JO - Carlsberg Research Communications

JF - Carlsberg Research Communications

IS - 3

ER -

A cDNA clone for protein Z, a major barley endosperm albumin

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