Variable glucosinolate profiles of Cardamine pratensis (Brassicaceae) with equal chromosome numbers

TY - JOUR

T1 - Variable glucosinolate profiles of Cardamine pratensis (Brassicaceae) with equal chromosome numbers

AU - Agerbirk, Niels

AU - Olsen, Carl Erik

AU - Chew, Frances S.

AU - Ørgaard, Marian

PY - 2010/4/28

Y1 - 2010/4/28

N2 - A novel glucosinolate, 3-(hydroxymethyl)pentylglucosinolate, was present at high levels in Cardamine pratensis L. from eastern North America and in commercially obtained seeds, but not in C. pratensis plants from southern Scandinavia. Glucosinolates in a number of accessions of C. pratensis included glucosinolates with the side chains 1-methylethyl, 1-(hydroxymethyl)ethyl, 1-methylpropyl, 1-(hydroxymethyl)propyl, 3-methylpentyl, 3-(hydroxymethyl) pentyl, benzyl, 4-hydroxybenzyl, 4-methoxybenzyl, indol-3-ylmethyl (as well as its 1-methoxy, 4-hydroxy, and 4-methoxy derivatives) and the rare side chain 1,4-dimethoxyindol-3-ylmethyl. Substantial variation was observed for four biosynthetic characters: (i) extent of chain elongation of Ile-derived glucosinolates; (ii) biosynthesis of Phe/Tyr-derived glucosinolates in general; (iii) hydroxylation of branched-chain glucosinolates; and (iv) O-methylation of 4-hydroxybenzylglucosinolate (sinalbin). Cytological analysis of pollen mother cells and root tip cells in meiosis and mitosis established the chromosome number to be 2n = 30 for all accessions, irrespective of glucosinolate profile.

AB - A novel glucosinolate, 3-(hydroxymethyl)pentylglucosinolate, was present at high levels in Cardamine pratensis L. from eastern North America and in commercially obtained seeds, but not in C. pratensis plants from southern Scandinavia. Glucosinolates in a number of accessions of C. pratensis included glucosinolates with the side chains 1-methylethyl, 1-(hydroxymethyl)ethyl, 1-methylpropyl, 1-(hydroxymethyl)propyl, 3-methylpentyl, 3-(hydroxymethyl) pentyl, benzyl, 4-hydroxybenzyl, 4-methoxybenzyl, indol-3-ylmethyl (as well as its 1-methoxy, 4-hydroxy, and 4-methoxy derivatives) and the rare side chain 1,4-dimethoxyindol-3-ylmethyl. Substantial variation was observed for four biosynthetic characters: (i) extent of chain elongation of Ile-derived glucosinolates; (ii) biosynthesis of Phe/Tyr-derived glucosinolates in general; (iii) hydroxylation of branched-chain glucosinolates; and (iv) O-methylation of 4-hydroxybenzylglucosinolate (sinalbin). Cytological analysis of pollen mother cells and root tip cells in meiosis and mitosis established the chromosome number to be 2n = 30 for all accessions, irrespective of glucosinolate profile.

U2 - 10.1021/jf904362m

DO - 10.1021/jf904362m

M3 - Journal article

C2 - 20334382

SN - 0021-8561

VL - 58

SP - 4693

EP - 4700

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 8

ER -