Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients

TY - JOUR

T1 - Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients

AU - Platzer, M H

AU - Grattan, C E H

AU - Poulsen, Lars K.

AU - Skov, P S

PY - 2005

Y1 - 2005

N2 - Endogenous histamine-releasing factors (HRFs) are involved in 30-60% of patients with chronic urticaria (CU). Evidence for their existence comes from in vivo studies of autoreactivity with the autologous serum skin test (ASST), in vitro immunoassays demonstrating autoantibodies against the immunoglobulin E (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine release (HR) from basophils and mast cells. We have examined the correlation between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria test) in a group of well-characterized CU patients and subsequently determined the frequency of HR-Urticaria-positive sera from a larger population of CU patients.

AB - Endogenous histamine-releasing factors (HRFs) are involved in 30-60% of patients with chronic urticaria (CU). Evidence for their existence comes from in vivo studies of autoreactivity with the autologous serum skin test (ASST), in vitro immunoassays demonstrating autoantibodies against the immunoglobulin E (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine release (HR) from basophils and mast cells. We have examined the correlation between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria test) in a group of well-characterized CU patients and subsequently determined the frequency of HR-Urticaria-positive sera from a larger population of CU patients.

KW - Adolescent

KW - Adult

KW - Aged

KW - Autoantibodies

KW - Basophils

KW - Child

KW - Child, Preschool

KW - Chronic Disease

KW - Female

KW - Histamine

KW - Humans

KW - Immunoassay

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Middle Aged

KW - Sensitivity and Specificity

KW - Skin Tests

KW - Urticaria

U2 - 10.1111/j.1398-9995.2005.00841.x

DO - 10.1111/j.1398-9995.2005.00841.x

M3 - Journal article

C2 - 16076300

SN - 0105-4538

VL - 60

SP - 1152

EP - 1156

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

IS - 9

ER -