Transcriptome complexity in a genome-reduced bacterium.

Marc Güell; Vera van Noort; Eva Yus; Wei-Hua Chen; Justine Leigh-Bell; Konstantinos Michalodimitrakis; Takuji Yamada; Manimozhiyan Arumugam; Tobias Doerks; Sebastian Kühner; Michaela Rode; Mikita Suyama; Sabine Schmidt; Anne-Claude Gavin; Peer Bork; Luis Serrano

doi:10.1126/science.1176951

Transcriptome complexity in a genome-reduced bacterium.

Marc Güell, Vera van Noort, Eva Yus, Wei-Hua Chen, Justine Leigh-Bell, Konstantinos Michalodimitrakis, Takuji Yamada, Manimozhiyan Arumugam, Tobias Doerks, Sebastian Kühner, Michaela Rode, Mikita Suyama, Sabine Schmidt, Anne-Claude Gavin, Peer Bork, Luis Serrano

324 Citationer (Scopus)

Abstract

To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

Originalsprog	Engelsk
Tidsskrift	Science
Vol/bind	326
Udgave nummer	5957
Sider (fra-til)	1268-1271
Antal sider	4
ISSN	0036-8075
DOI	https://doi.org/10.1126/science.1176951
Status	Udgivet - nov. 2009

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10.1126/science.1176951

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title = "Transcriptome complexity in a genome-reduced bacterium.",

abstract = "To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.",

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author = "Marc G{\"u}ell and {van Noort}, Vera and Eva Yus and Wei-Hua Chen and Justine Leigh-Bell and Konstantinos Michalodimitrakis and Takuji Yamada and Manimozhiyan Arumugam and Tobias Doerks and Sebastian K{\"u}hner and Michaela Rode and Mikita Suyama and Sabine Schmidt and Anne-Claude Gavin and Peer Bork and Luis Serrano",

year = "2009",

month = nov,

doi = "10.1126/science.1176951",

language = "English",

volume = "326",

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TY - JOUR

T1 - Transcriptome complexity in a genome-reduced bacterium.

AU - Güell, Marc

AU - van Noort, Vera

AU - Yus, Eva

AU - Chen, Wei-Hua

AU - Leigh-Bell, Justine

AU - Michalodimitrakis, Konstantinos

AU - Yamada, Takuji

AU - Arumugam, Manimozhiyan

AU - Doerks, Tobias

AU - Kühner, Sebastian

AU - Rode, Michaela

AU - Suyama, Mikita

AU - Schmidt, Sabine

AU - Gavin, Anne-Claude

AU - Bork, Peer

AU - Serrano, Luis

PY - 2009/11

Y1 - 2009/11

N2 - To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

AB - To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

KW - Antisense

KW - Antisense: genetics

KW - Antisense: metabolism

KW - Bacterial

KW - Bacterial: genetics

KW - Bacterial: metabolism

KW - Base Sequence

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Genes

KW - Genetic

KW - Genome

KW - Messenger

KW - Messenger: genetics

KW - Messenger: metabolism

KW - Molecular Sequence Data

KW - Mycoplasma pneumoniae

KW - Mycoplasma pneumoniae: genetics

KW - Mycoplasma pneumoniae: metabolism

KW - Oligonucleotide Array Sequence Analysis

KW - Operon

KW - RNA

KW - Transcription

KW - Untranslated

KW - Untranslated: analysis

KW - Untranslated: genetics

U2 - 10.1126/science.1176951

DO - 10.1126/science.1176951

M3 - Journal article

SN - 0036-8075

VL - 326

SP - 1268

EP - 1271

JO - Science

JF - Science

IS - 5957

ER -

Transcriptome complexity in a genome-reduced bacterium.

Abstract

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