The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes

Stefan Peter Mortensen; Kamilla Munch Winding; Ulrik Winning Iepsen; Gregers Winding Munch; Niels Marcussen; Ylva Hellsten; Bente Klarlund Pedersen; Oliver Baum

doi:10.1111/sms.13348

The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes

Stefan Peter Mortensen, Kamilla Munch Winding, Ulrik Winning Iepsen, Gregers Winding Munch, Niels Marcussen, Ylva Hellsten, Bente Klarlund Pedersen, Oliver Baum

August Krogh Sektionen for Human Fysiologi

8 Citationer (Scopus)

Abstract

Type 2 diabetes is associated with microvascular dysfunction, but little is known about how capillary ultrastructure is affected by exercise training. To investigate the effect of two types of exercise training on skeletal muscle capillary ultrastructure and capillarization in individuals with type 2 diabetes, 21 individuals with type 2 diabetes were allocated (randomized controlled trial) to 11 weeks of aerobic exercise training consisting of either moderate-intensity endurance training (END; n = 10) or low-volume high-intensity interval training (HIIT; n = 11). Skeletal muscle biopsies (m vastus lateralis) were obtained before and after the training intervention. At baseline, there was no difference in capillarization, capillary structure, and exercise hyperemia between the two groups. After the training intervention, capillary-to-fiber ratio increased by 8% ± 3% in the END group (P < 0.05) and was unchanged in the HIIT group with no difference between groups. Endothelium thickness increased (P < 0.05), basement membrane thickness decreased (P < 0.05), and the capillary lumen tended (P = 0.07) to increase in the END group, whereas these structural indicators were unchanged after HIIT. In contrast, skeletal muscle endothelial nitric oxide synthase (eNOS) increased after HIIT (P < 0.05), but not END, whereas there was no change in vascular endothelial growth factor (VEGF), superoxide dismutase (SOD)-2, or NADPH oxidase after both training protocols. In contrast to END training, HIIT did not alter capillarization or capillary structure in individuals with type 2 diabetes. In conclusion, HIIT appears to be a less effective strategy to treat capillary rarefaction and reduce basement thickening in type 2 diabetes.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Medicine & Science in Sports
Vol/bind	29
Udgave nummer	3
Sider (fra-til)	360-368
Antal sider	9
ISSN	0905-7188
DOI	https://doi.org/10.1111/sms.13348
Status	Udgivet - mar. 2019

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10.1111/sms.13348

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The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes. / Mortensen, Stefan Peter; Winding, Kamilla Munch; Iepsen, Ulrik Winning et al.
I: Scandinavian Journal of Medicine & Science in Sports, Bind 29, Nr. 3, 03.2019, s. 360-368.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes",

abstract = "Type 2 diabetes is associated with microvascular dysfunction, but little is known about how capillary ultrastructure is affected by exercise training. To investigate the effect of two types of exercise training on skeletal muscle capillary ultrastructure and capillarization in individuals with type 2 diabetes, 21 individuals with type 2 diabetes were allocated (randomized controlled trial) to 11 weeks of aerobic exercise training consisting of either moderate-intensity endurance training (END; n = 10) or low-volume high-intensity interval training (HIIT; n = 11). Skeletal muscle biopsies (m vastus lateralis) were obtained before and after the training intervention. At baseline, there was no difference in capillarization, capillary structure, and exercise hyperemia between the two groups. After the training intervention, capillary-to-fiber ratio increased by 8% ± 3% in the END group (P < 0.05) and was unchanged in the HIIT group with no difference between groups. Endothelium thickness increased (P < 0.05), basement membrane thickness decreased (P < 0.05), and the capillary lumen tended (P = 0.07) to increase in the END group, whereas these structural indicators were unchanged after HIIT. In contrast, skeletal muscle endothelial nitric oxide synthase (eNOS) increased after HIIT (P < 0.05), but not END, whereas there was no change in vascular endothelial growth factor (VEGF), superoxide dismutase (SOD)-2, or NADPH oxidase after both training protocols. In contrast to END training, HIIT did not alter capillarization or capillary structure in individuals with type 2 diabetes. In conclusion, HIIT appears to be a less effective strategy to treat capillary rarefaction and reduce basement thickening in type 2 diabetes.",

keywords = "Faculty of Science, Endothelium, Capillary ultrastructure, Microcirculation, Skeletal muscle, Exercise",

author = "Mortensen, {Stefan Peter} and Winding, {Kamilla Munch} and Iepsen, {Ulrik Winning} and Munch, {Gregers Winding} and Niels Marcussen and Ylva Hellsten and Pedersen, {Bente Klarlund} and Oliver Baum",

note = "CURIS 2019 NEXS 017",

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month = mar,

doi = "10.1111/sms.13348",

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T1 - The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes

AU - Mortensen, Stefan Peter

AU - Winding, Kamilla Munch

AU - Iepsen, Ulrik Winning

AU - Munch, Gregers Winding

AU - Marcussen, Niels

AU - Hellsten, Ylva

AU - Pedersen, Bente Klarlund

AU - Baum, Oliver

N1 - CURIS 2019 NEXS 017

PY - 2019/3

Y1 - 2019/3

N2 - Type 2 diabetes is associated with microvascular dysfunction, but little is known about how capillary ultrastructure is affected by exercise training. To investigate the effect of two types of exercise training on skeletal muscle capillary ultrastructure and capillarization in individuals with type 2 diabetes, 21 individuals with type 2 diabetes were allocated (randomized controlled trial) to 11 weeks of aerobic exercise training consisting of either moderate-intensity endurance training (END; n = 10) or low-volume high-intensity interval training (HIIT; n = 11). Skeletal muscle biopsies (m vastus lateralis) were obtained before and after the training intervention. At baseline, there was no difference in capillarization, capillary structure, and exercise hyperemia between the two groups. After the training intervention, capillary-to-fiber ratio increased by 8% ± 3% in the END group (P < 0.05) and was unchanged in the HIIT group with no difference between groups. Endothelium thickness increased (P < 0.05), basement membrane thickness decreased (P < 0.05), and the capillary lumen tended (P = 0.07) to increase in the END group, whereas these structural indicators were unchanged after HIIT. In contrast, skeletal muscle endothelial nitric oxide synthase (eNOS) increased after HIIT (P < 0.05), but not END, whereas there was no change in vascular endothelial growth factor (VEGF), superoxide dismutase (SOD)-2, or NADPH oxidase after both training protocols. In contrast to END training, HIIT did not alter capillarization or capillary structure in individuals with type 2 diabetes. In conclusion, HIIT appears to be a less effective strategy to treat capillary rarefaction and reduce basement thickening in type 2 diabetes.

AB - Type 2 diabetes is associated with microvascular dysfunction, but little is known about how capillary ultrastructure is affected by exercise training. To investigate the effect of two types of exercise training on skeletal muscle capillary ultrastructure and capillarization in individuals with type 2 diabetes, 21 individuals with type 2 diabetes were allocated (randomized controlled trial) to 11 weeks of aerobic exercise training consisting of either moderate-intensity endurance training (END; n = 10) or low-volume high-intensity interval training (HIIT; n = 11). Skeletal muscle biopsies (m vastus lateralis) were obtained before and after the training intervention. At baseline, there was no difference in capillarization, capillary structure, and exercise hyperemia between the two groups. After the training intervention, capillary-to-fiber ratio increased by 8% ± 3% in the END group (P < 0.05) and was unchanged in the HIIT group with no difference between groups. Endothelium thickness increased (P < 0.05), basement membrane thickness decreased (P < 0.05), and the capillary lumen tended (P = 0.07) to increase in the END group, whereas these structural indicators were unchanged after HIIT. In contrast, skeletal muscle endothelial nitric oxide synthase (eNOS) increased after HIIT (P < 0.05), but not END, whereas there was no change in vascular endothelial growth factor (VEGF), superoxide dismutase (SOD)-2, or NADPH oxidase after both training protocols. In contrast to END training, HIIT did not alter capillarization or capillary structure in individuals with type 2 diabetes. In conclusion, HIIT appears to be a less effective strategy to treat capillary rarefaction and reduce basement thickening in type 2 diabetes.

KW - Faculty of Science

KW - Endothelium

KW - Capillary ultrastructure

KW - Microcirculation

KW - Skeletal muscle

KW - Exercise

U2 - 10.1111/sms.13348

DO - 10.1111/sms.13348

M3 - Journal article

C2 - 30480353

SN - 0905-7188

VL - 29

SP - 360

EP - 368

JO - Scandinavian Journal of Medicine & Science in Sports

JF - Scandinavian Journal of Medicine & Science in Sports

IS - 3

ER -

The effect of two exercise modalities on skeletal muscle capillary ultrastructure in individuals with type 2 diabetes

Abstract

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