Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial

Gabriella Christina van Zanten; Lukasz Krych; Henna Röytiö; Sofia Forssten; Sampo J. Lahtinen; Waleed Abu Al-Soud; Søren Johannes Sørensen; Birte Svensson; Lene Jespersen; Mogens Jakobsen

doi:10.1111/1574-6941.12397

Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial

Gabriella Christina van Zanten, Lukasz Krych, Henna Röytiö, Sofia Forssten, Sampo J. Lahtinen, Waleed Abu Al-Soud, Søren Johannes Sørensen, Birte Svensson, Lene Jespersen, Mogens Jakobsen

32 Citationer (Scopus)

Abstract

Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n = 18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (10⁹ CFU) and cellobiose (5 g)] or placebo daily for 3 weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P < 0.05). No other effects were found on microbiota composition. Remarkably, however, the synbiotic increased concentrations of branched-chain fatty acids, measured by gas chromatography, while short-chain fatty acids were not affected.

Originalsprog	Engelsk
Tidsskrift	F E M S Microbiology Ecology
Vol/bind	90
Udgave nummer	1
Sider (fra-til)	225-236
Antal sider	12
ISSN	0168-6496
DOI	https://doi.org/10.1111/1574-6941.12397
Status	Udgivet - 1 okt. 2014

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10.1111/1574-6941.12397

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Citationsformater

van Zanten, G. C., Krych, L., Röytiö, H., Forssten, S., Lahtinen, S. J., Abu Al-Soud, W., Sørensen, S. J., Svensson, B., Jespersen, L., & Jakobsen, M. (2014). Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. F E M S Microbiology Ecology, 90(1), 225-236. https://doi.org/10.1111/1574-6941.12397

Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids : a randomized, double-blinded cross-over trial. / van Zanten, Gabriella Christina; Krych, Lukasz; Röytiö, Henna et al.

I: F E M S Microbiology Ecology, Bind 90, Nr. 1, 01.10.2014, s. 225-236.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

van Zanten, GC, Krych, L, Röytiö, H, Forssten, S, Lahtinen, SJ, Abu Al-Soud, W, Sørensen, SJ, Svensson, B, Jespersen, L & Jakobsen, M 2014, 'Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial', F E M S Microbiology Ecology, bind 90, nr. 1, s. 225-236. https://doi.org/10.1111/1574-6941.12397

van Zanten GC, Krych L, Röytiö H, Forssten S, Lahtinen SJ, Abu Al-Soud W et al. Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial. F E M S Microbiology Ecology. 2014 okt. 1;90(1):225-236. doi: 10.1111/1574-6941.12397

van Zanten, Gabriella Christina ; Krych, Lukasz ; Röytiö, Henna et al. / Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids : a randomized, double-blinded cross-over trial. I: F E M S Microbiology Ecology. 2014 ; Bind 90, Nr. 1. s. 225-236.

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abstract = "Probiotics, prebiotics, and combinations thereof, that is synbiotics, have been reported to modulate gut microbiota of humans. In this study, effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n = 18) were enrolled in a double-blinded, randomized, and placebo-controlled cross-over study and received synbiotic [Lactobacillus acidophilus NCFM (109 CFU) and cellobiose (5 g)] or placebo daily for 3 weeks. Fecal samples were collected and lactobacilli numbers were quantified by qPCR. Furthermore, 454 tag-encoded amplicon pyrosequencing was used to monitor the effect of synbiotic on the composition of the microbiota. The synbiotic increased levels of Lactobacillus spp. and relative abundances of the genera Bifidobacterium, Collinsella, and Eubacterium while the genus Dialister was decreased (P < 0.05). No other effects were found on microbiota composition. Remarkably, however, the synbiotic increased concentrations of branched-chain fatty acids, measured by gas chromatography, while short-chain fatty acids were not affected.",

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