Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation

TY - JOUR

T1 - Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation

AU - Leijon, M

AU - Gräslund, A

AU - Buchardt, O

AU - Nordén, B

AU - Eriksson, M

AU - Nielsen, Peter E.

PY - 1994/8/23

Y1 - 1994/8/23

N2 - The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

AB - The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

KW - Base Composition

KW - Base Sequence

KW - DNA/chemistry

KW - Hydrogen Bonding

KW - Magnetic Resonance Spectroscopy

KW - Molecular Sequence Data

KW - Nucleic Acid Conformation

KW - Nucleic Acid Hybridization

KW - Nucleic Acids/chemistry

KW - Protons

M3 - Journal article

C2 - 8060989

SN - 0006-2960

VL - 33

SP - 9820

EP - 9825

JO - Biochemistry

JF - Biochemistry

IS - 33

ER -