Specific proliferative response of human lymphocytes to purified soluble antigens from Plasmodium falciparum in vitro cultures and to antigens from malaria patients' sera

TY - JOUR

T1 - Specific proliferative response of human lymphocytes to purified soluble antigens from Plasmodium falciparum in vitro cultures and to antigens from malaria patients' sera

AU - Bygbjerg, I C

AU - Jepsen, S

AU - Theander, T G

AU - Odum, Niels

N1 - Keywords: Adult; Antigens, Protozoan; Cells, Cultured; Female; Humans; Immunoglobulin G; Lymphocyte Activation; Lymphocytes; Malaria; Male; Plasmodium falciparum; Solubility; Thymidine; Time Factors

PY - 1985

Y1 - 1985

N2 - Antigens of Plasmodium falciparum, in supernatants of in vitro cultures of the parasite were affinity purified on columns prepared with the IgG fraction of the serum of an immune individual. The purified antigens induced proliferation of lymphocytes from persons who had recently had malaria. The responses were strongest with lymphocytes from individuals infected with falciparum and ovale malaria; vivax malaria infections induced a lower level of response and lymphocytes of unsensitized individuals were little affected. Lymphocytes from unsensitized individuals did not respond to the affinity purified antigen preparations from malaria patients' sera indicating that significant amounts of non-specific mitogens were not present.

AB - Antigens of Plasmodium falciparum, in supernatants of in vitro cultures of the parasite were affinity purified on columns prepared with the IgG fraction of the serum of an immune individual. The purified antigens induced proliferation of lymphocytes from persons who had recently had malaria. The responses were strongest with lymphocytes from individuals infected with falciparum and ovale malaria; vivax malaria infections induced a lower level of response and lymphocytes of unsensitized individuals were little affected. Lymphocytes from unsensitized individuals did not respond to the affinity purified antigen preparations from malaria patients' sera indicating that significant amounts of non-specific mitogens were not present.

M3 - Journal article

C2 - 3884198

SN - 0009-9104

VL - 59

SP - 421

EP - 426

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

IS - 2

ER -