TY - JOUR
T1 - Skin reaction and regeneration after single sodium lauryl sulfate exposure stratified by filaggrin genotype and atopic dermatitis phenotype
AU - Bandier, Josefine
AU - Carlsen, B.C.
AU - Rasmussen, Morten Arendt
AU - Petersen, L.J.
AU - Johansen, J.D.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Background Filaggrin is key for the integrity of the stratum corneum. Mutations in the filaggrin gene (FLGnull) play a prominent role in atopic dermatitis (AD) pathogenesis. People with AD have increased susceptibility to irritants. However, little is known about the effect of filaggrin genotype and AD phenotype on irritant response and skin regeneration. Objectives To investigate the role of FLGnull and AD groups for skin reaction and recovery after sodium lauryl sulfate (SLS) irritation. Methods This is a case-control study comprising 67 subjects, including healthy controls and patients with and without FLGnull and AD. Reactivity to different doses of SLS at 24, 48, 72 and 145 h after SLS application was measured by transepidermal water loss (TEWL) and laser Doppler flowmetry (LDF). Reactivity was assessed univariately and by pattern analysis. Results All patient groups showed a higher degree of skin-barrier disruption and inflammation than did controls in response to SLS. Assessing reactivity by the delta value of the area under the curve for both TEWL and LDF showed significant differences between healthy controls and those with the AD phenotype, irrespective of filaggrin mutation. The poorest regeneration was among those with the AD phenotype. The two AD phenotype groups were separated by multivariate technique, due to earlier inflammatory reactivity among subjects with FLGnullplusAD compared with the AD phenotype alone. Conclusions Both skin reaction and regeneration were significantly different between the patient population and the healthy controls. Additionally, response severity and regeneration depended more on AD phenotype than on filaggrin genotype, whereas the response was more rapid among the FLGnullplusAD individuals. What's already known about this topic? Subjects with atopic dermatitis (AD) have barrier defects and increased susceptibility to irritants. Studies have suggested that filaggrin mutations facilitate penetration of irritants, which could explain the increased risk of irritant contact dermatitis among patients with AD and filaggrin mutations. What does this study add? Skin response and regeneration after sodium lauryl sulfate barrier disruption are more dependent on AD than on filaggrin genotype. Those with both mutations and AD have higher inflammatory alertness, with a more rapid inflammatory response.
AB - Background Filaggrin is key for the integrity of the stratum corneum. Mutations in the filaggrin gene (FLGnull) play a prominent role in atopic dermatitis (AD) pathogenesis. People with AD have increased susceptibility to irritants. However, little is known about the effect of filaggrin genotype and AD phenotype on irritant response and skin regeneration. Objectives To investigate the role of FLGnull and AD groups for skin reaction and recovery after sodium lauryl sulfate (SLS) irritation. Methods This is a case-control study comprising 67 subjects, including healthy controls and patients with and without FLGnull and AD. Reactivity to different doses of SLS at 24, 48, 72 and 145 h after SLS application was measured by transepidermal water loss (TEWL) and laser Doppler flowmetry (LDF). Reactivity was assessed univariately and by pattern analysis. Results All patient groups showed a higher degree of skin-barrier disruption and inflammation than did controls in response to SLS. Assessing reactivity by the delta value of the area under the curve for both TEWL and LDF showed significant differences between healthy controls and those with the AD phenotype, irrespective of filaggrin mutation. The poorest regeneration was among those with the AD phenotype. The two AD phenotype groups were separated by multivariate technique, due to earlier inflammatory reactivity among subjects with FLGnullplusAD compared with the AD phenotype alone. Conclusions Both skin reaction and regeneration were significantly different between the patient population and the healthy controls. Additionally, response severity and regeneration depended more on AD phenotype than on filaggrin genotype, whereas the response was more rapid among the FLGnullplusAD individuals. What's already known about this topic? Subjects with atopic dermatitis (AD) have barrier defects and increased susceptibility to irritants. Studies have suggested that filaggrin mutations facilitate penetration of irritants, which could explain the increased risk of irritant contact dermatitis among patients with AD and filaggrin mutations. What does this study add? Skin response and regeneration after sodium lauryl sulfate barrier disruption are more dependent on AD than on filaggrin genotype. Those with both mutations and AD have higher inflammatory alertness, with a more rapid inflammatory response.
U2 - 10.1111/bjd.13651
DO - 10.1111/bjd.13651
M3 - Journal article
C2 - 25581911
SN - 0007-0963
VL - 172
SP - 1519
EP - 1529
JO - British Journal of Dermatology
JF - British Journal of Dermatology
IS - 6
ER -