Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3

TY - JOUR

T1 - Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3

AU - Mankouri, Hocine W

AU - Ngo, Hien-Ping

AU - Hickson, Ian D

PY - 2007/10/1

Y1 - 2007/10/1

N2 - CSM2, PSY3, SHU1, and SHU2 (collectively referred to as the SHU genes) were identified in Saccharomyces cerevisiae as four genes in the same epistasis group that suppress various sgs1 and top3 mutant phenotypes when mutated. Although the SHU genes have been implicated in homologous recombination repair (HRR), their precise role(s) within this pathway remains poorly understood. Here, we have identified a specific role for the Shu proteins in a Rad51/Rad54-dependent HRR pathway(s) to repair MMS-induced lesions during S-phase. We show that, although mutation of RAD51 or RAD54 prevented the formation of MMS-induced HRR intermediates (X-molecules) arising during replication in sgs1 cells, mutation of SHU genes attenuated the level of these structures. Similar findings were also observed in shu1 cells in which Rmi1 or Top3 function was impaired. We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex.

AB - CSM2, PSY3, SHU1, and SHU2 (collectively referred to as the SHU genes) were identified in Saccharomyces cerevisiae as four genes in the same epistasis group that suppress various sgs1 and top3 mutant phenotypes when mutated. Although the SHU genes have been implicated in homologous recombination repair (HRR), their precise role(s) within this pathway remains poorly understood. Here, we have identified a specific role for the Shu proteins in a Rad51/Rad54-dependent HRR pathway(s) to repair MMS-induced lesions during S-phase. We show that, although mutation of RAD51 or RAD54 prevented the formation of MMS-induced HRR intermediates (X-molecules) arising during replication in sgs1 cells, mutation of SHU genes attenuated the level of these structures. Similar findings were also observed in shu1 cells in which Rmi1 or Top3 function was impaired. We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex.

KW - Cell Proliferation

KW - DNA Repair

KW - DNA-Binding Proteins

KW - Epistasis, Genetic

KW - Genes, Fungal

KW - Hydroxyurea

KW - Methyl Methanesulfonate

KW - Models, Biological

KW - Mutant Proteins

KW - Mutation

KW - RecQ Helicases

KW - Recombination, Genetic

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

U2 - 10.1091/mbc.E07-05-0490

DO - 10.1091/mbc.E07-05-0490

M3 - Journal article

C2 - 17671161

SN - 1059-1524

VL - 18

SP - 4062

EP - 4073

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

IS - 10

ER -