Sequence specific inhibition of DNA restriction enzyme cleavage by PNA

TY - JOUR

T1 - Sequence specific inhibition of DNA restriction enzyme cleavage by PNA

AU - Buchardt, O

AU - Nielsen, Peter E.

PY - 1993/1/25

Y1 - 1993/1/25

N2 - Plasmids containing double-stranded 10-mer PNA (peptide nucleic acid chimera) targets proximally flanked by two restriction enzyme sites were challenged with the complementary PNA or PNAs having one or two mismatches, and the effect on the restriction enzyme cleavage of the flanking sites was assayed. The following PNAs were used: T10-LysNH2, T5CT4-LysNH2 and T2CT2CT4-LysNH2 and the corresponding targets cloned into pUC 19 were flanked by BamH1, Sal1 or Pstl sites, respectively. In all cases it was found that complete inhibition of restriction enzyme cleavage was obtained with the complementary PNA, a significantly reduced effect was seen with a PNA having one mismatch, and no effect was seen with a PNA having two mismatches. These results show that PNA can be used as sequence specific blockers of DNA recognizing proteins.

AB - Plasmids containing double-stranded 10-mer PNA (peptide nucleic acid chimera) targets proximally flanked by two restriction enzyme sites were challenged with the complementary PNA or PNAs having one or two mismatches, and the effect on the restriction enzyme cleavage of the flanking sites was assayed. The following PNAs were used: T10-LysNH2, T5CT4-LysNH2 and T2CT2CT4-LysNH2 and the corresponding targets cloned into pUC 19 were flanked by BamH1, Sal1 or Pstl sites, respectively. In all cases it was found that complete inhibition of restriction enzyme cleavage was obtained with the complementary PNA, a significantly reduced effect was seen with a PNA having one mismatch, and no effect was seen with a PNA having two mismatches. These results show that PNA can be used as sequence specific blockers of DNA recognizing proteins.

KW - Base Sequence

KW - Chimera

KW - DNA

KW - DNA Restriction Enzymes/antagonists & inhibitors

KW - Deoxyribonuclease BamHI/antagonists & inhibitors

KW - Deoxyribonucleases, Type II Site-Specific/antagonists & inhibitors

KW - Molecular Sequence Data

KW - Oligodeoxyribonucleotides/metabolism

KW - Peptides/metabolism

KW - Restriction Mapping

M3 - Journal article

C2 - 8382793

SN - 0305-1048

VL - 21

SP - 197

EP - 200

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 2

ER -