Sequence selective double strand DNA cleavage by peptide nucleic acid (PNA) targeting using nuclease S1

TY - JOUR

T1 - Sequence selective double strand DNA cleavage by peptide nucleic acid (PNA) targeting using nuclease S1

AU - Demidov, V

AU - Frank-Kamenetskii, M D

AU - Buchardt, O

AU - Nielsen, Peter E.

PY - 1993/5/11

Y1 - 1993/5/11

N2 - A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtained. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site is targeted, and additionally enhanced if this site is in trans rather than in cis orientation. Thus in effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

AB - A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtained. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site is targeted, and additionally enhanced if this site is in trans rather than in cis orientation. Thus in effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

KW - Base Sequence

KW - DNA/metabolism

KW - Genetic Techniques

KW - Kinetics

KW - Molecular Sequence Data

KW - Oligodeoxyribonucleotides/chemical synthesis

KW - Single-Strand Specific DNA and RNA Endonucleases/metabolism

KW - Substrate Specificity

M3 - Journal article

C2 - 8502550

SN - 0305-1048

VL - 21

SP - 2103

EP - 2107

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 9

ER -