Separation of fat-soluble vitamins by hydrophobic interaction electrokinetic chromatography with tetradecylammonium ions as pseudostationary phase

TY - JOUR

T1 - Separation of fat-soluble vitamins by hydrophobic interaction electrokinetic chromatography with tetradecylammonium ions as pseudostationary phase

AU - Pedersen-Bjergaard, Stig

AU - Einar Rasmussen, Knut

AU - Tilander, Tuija

PY - 1998/5/22

Y1 - 1998/5/22

N2 - Hydrophobic interaction electrokinetic chromatography (HIEKC) was evaluated for the separation of nonionic hydrophobic pharmaceuticals utilizing vitamin A palmitate, vitamin E acetate, and vitamin D3 as model compounds. In order to effectively dissolve the highly hydrophobic vitamins, the separation medium consisted of acetonitrile-water (80:20, v/v). Tetradecylammonium (TDA+) at a concentration of 80 mM was added to the separation medium as pseudostationary phase, and the three vitamins were separated prior to the electroosmotic flow based on different hydrophobic interactions to the charged TDA+ molecules. With a 47 cmx50 μm internal diameter uncoated fused-silica capillary, the three vitamins were baseline separated within 6 min utilizing a separation voltage of 10 kV. Migration times were repeatable within 1.3% and varied less than 4.2% between 5 different days. Peak areas were repeatable within 1.7% and varied less than 4.2% between 5 different days.

AB - Hydrophobic interaction electrokinetic chromatography (HIEKC) was evaluated for the separation of nonionic hydrophobic pharmaceuticals utilizing vitamin A palmitate, vitamin E acetate, and vitamin D3 as model compounds. In order to effectively dissolve the highly hydrophobic vitamins, the separation medium consisted of acetonitrile-water (80:20, v/v). Tetradecylammonium (TDA+) at a concentration of 80 mM was added to the separation medium as pseudostationary phase, and the three vitamins were separated prior to the electroosmotic flow based on different hydrophobic interactions to the charged TDA+ molecules. With a 47 cmx50 μm internal diameter uncoated fused-silica capillary, the three vitamins were baseline separated within 6 min utilizing a separation voltage of 10 kV. Migration times were repeatable within 1.3% and varied less than 4.2% between 5 different days. Peak areas were repeatable within 1.7% and varied less than 4.2% between 5 different days.

KW - Hydrophobic interaction electrokinetic chromatography

KW - Pseudostationary phases

KW - Tetradecylammonium ions

KW - Vitamins

UR - http://www.scopus.com/inward/record.url?scp=0032557591&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(98)00087-9

DO - 10.1016/S0021-9673(98)00087-9

M3 - Journal article

AN - SCOPUS:0032557591

SN - 0301-4770

VL - 807

SP - 285

EP - 295

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 2

ER -