Seagrass rhizosphere microenvironment alters plant-associated microbial community composition

Kasper Elgetti Brodersen; Nashshon Siboni; Daniel A. Nielsen; Mathieu Pernice; Peter J. Ralph; Justin Seymour; Michael Kühl

doi:10.1111/1462-2920.14245

Seagrass rhizosphere microenvironment alters plant-associated microbial community composition

Kasper Elgetti Brodersen, Nashshon Siboni, Daniel A. Nielsen, Mathieu Pernice, Peter J. Ralph, Justin Seymour, Michael Kühl

Marine Biological Section

27 Citationer (Scopus)

Abstract

The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O₂ and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM). Radial O₂ loss (ROL) from the BLM led to ∼ 300 µm thick oxic microzones, wherein pronounced decreases in H₂S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; ∼ 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H₂S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.

Originalsprog	Engelsk
Tidsskrift	Environmental Microbiology
Vol/bind	20
Udgave nummer	8
Sider (fra-til)	2854-2864
ISSN	1462-2912
DOI	https://doi.org/10.1111/1462-2920.14245
Status	Udgivet - aug. 2018

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10.1111/1462-2920.14245

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title = "Seagrass rhizosphere microenvironment alters plant-associated microbial community composition",

abstract = "The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O2 and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM). Radial O2 loss (ROL) from the BLM led to ∼ 300 µm thick oxic microzones, wherein pronounced decreases in H2S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; ∼ 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H2S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.",

author = "Brodersen, {Kasper Elgetti} and Nashshon Siboni and Nielsen, {Daniel A.} and Mathieu Pernice and Ralph, {Peter J.} and Justin Seymour and Michael K{\"u}hl",

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doi = "10.1111/1462-2920.14245",

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AU - Brodersen, Kasper Elgetti

AU - Siboni, Nashshon

AU - Nielsen, Daniel A.

AU - Pernice, Mathieu

AU - Ralph, Peter J.

AU - Seymour, Justin

AU - Kühl, Michael

PY - 2018/8

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N2 - The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O2 and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM). Radial O2 loss (ROL) from the BLM led to ∼ 300 µm thick oxic microzones, wherein pronounced decreases in H2S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; ∼ 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H2S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.

AB - The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O2 and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM). Radial O2 loss (ROL) from the BLM led to ∼ 300 µm thick oxic microzones, wherein pronounced decreases in H2S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; ∼ 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H2S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.

U2 - 10.1111/1462-2920.14245

DO - 10.1111/1462-2920.14245

M3 - Journal article

C2 - 29687545

SN - 1462-2912

VL - 20

SP - 2854

EP - 2864

JO - Environmental Microbiology

JF - Environmental Microbiology

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Seagrass rhizosphere microenvironment alters plant-associated microbial community composition

Abstract

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