RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

Vibe Hallundbæk Østergaard; Constanze Pentzold; Rune Troelsgaard Pedersen; Silviu Iosif; Arno Alpi; Simon Bekker-Jensen; Niels Mailand; Michael Lisby

doi:10.1016/j.dnarep.2012.08.005

RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

Vibe Hallundbæk Østergaard, Constanze Pentzold, Rune Troelsgaard Pedersen, Silviu Iosif, Arno Alpi, Simon Bekker-Jensen, Niels Mailand, Michael Lisby

20 Citationer (Scopus)

Abstract

The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.

Originalsprog	Engelsk
Tidsskrift	D N A Repair
Vol/bind	11
Udgave nummer	11
Sider (fra-til)	892–905
Antal sider	14
ISSN	1568-7864
DOI	https://doi.org/10.1016/j.dnarep.2012.08.005
Status	Udgivet - 1 nov. 2012

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10.1016/j.dnarep.2012.08.005

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title = "RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells",

abstract = "The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.",

author = "{\O}stergaard, {Vibe Hallundb{\ae}k} and Constanze Pentzold and Pedersen, {Rune Troelsgaard} and Silviu Iosif and Arno Alpi and Simon Bekker-Jensen and Niels Mailand and Michael Lisby",

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T1 - RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

AU - Østergaard, Vibe Hallundbæk

AU - Pentzold, Constanze

AU - Pedersen, Rune Troelsgaard

AU - Iosif, Silviu

AU - Alpi, Arno

AU - Bekker-Jensen, Simon

AU - Mailand, Niels

AU - Lisby, Michael

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.

AB - The ubiquitylation cascade plays an important role in the recruitment of repair factors at DNA double-strand breaks. The involvement of a growing number of ubiquitin E3 ligases adds to the complexity of the DNA damage-induced ubiquitin signaling. Here we use the genetically tractable avian cell line DT40 to investigate the role of HERC2, RNF8 and RNF168 in the DNA damage-induced ubiquitylation pathway. We show that formation of ubiquitin foci as well as cell survival after DNA damage depends on both RNF8 and RNF168. However, we find that RNF8 and RNF168 knockout cell lines respond differently to treatment with camptothecin indicating that they do not function in a strictly linear manner. Surprisingly, we show that HERC2 is required neither for survival nor for ubiquitin foci formation after DNA damage in DT40. Moreover, the E3 ubiquitin ligase activity of HERC2 is not redundant to that of RNF8 or RNF168.

U2 - 10.1016/j.dnarep.2012.08.005

DO - 10.1016/j.dnarep.2012.08.005

M3 - Journal article

C2 - 23010445

SN - 1568-7864

VL - 11

SP - 892

EP - 905

JO - D N A Repair

JF - D N A Repair

IS - 11

ER -

RNF8 and RNF168 but not HERC2 are required for DNA damage-induced ubiquitylation in chicken DT40 cells

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