Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA

TY - JOUR

T1 - Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA

AU - Ge, Wanzhong

AU - Deng, Qiannan

AU - Guo, Ting

AU - Hong, Xin

AU - Kugler, Jan-Michael

AU - Yang, Xiaohang

AU - Cohen, Stephen M

N1 - Copyright © 2015, The Genetics Society of America.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Pattern formation during epithelial development requires the coordination of multiple signaling pathways. Here, we investigate the functions of an ovary-enriched miRNA, miR-318, in epithelial development during Drosophila oogenesis. mir-318 maternal loss-of-function mutants were female-sterile and laid eggs with abnormal morphology. Removal of mir-318 disrupted the dorsal–anterior follicle cell patterning, resulting in abnormal dorsal appendages. mir-318 mutant females also produced thin and fragile eggshells due to impaired chorion gene amplification. We provide evidence that the ecdysone signaling pathway activates expression of miR-318 and that miR-318 cooperates with Tramtrack69 to control the switch from endocycling to chorion gene amplification during differentiation of the follicular epithelium. The multiple functions of miR-318 in oogenesis illustrate the importance of miRNAs in maintaining cell fate and in promoting the developmental transition in the female follicular epithelium.

AB - Pattern formation during epithelial development requires the coordination of multiple signaling pathways. Here, we investigate the functions of an ovary-enriched miRNA, miR-318, in epithelial development during Drosophila oogenesis. mir-318 maternal loss-of-function mutants were female-sterile and laid eggs with abnormal morphology. Removal of mir-318 disrupted the dorsal–anterior follicle cell patterning, resulting in abnormal dorsal appendages. mir-318 mutant females also produced thin and fragile eggshells due to impaired chorion gene amplification. We provide evidence that the ecdysone signaling pathway activates expression of miR-318 and that miR-318 cooperates with Tramtrack69 to control the switch from endocycling to chorion gene amplification during differentiation of the follicular epithelium. The multiple functions of miR-318 in oogenesis illustrate the importance of miRNAs in maintaining cell fate and in promoting the developmental transition in the female follicular epithelium.

U2 - 10.1534/genetics.115.174748

DO - 10.1534/genetics.115.174748

M3 - Journal article

C2 - 25786856

SN - 1943-2631

VL - 200

SP - 255

EP - 265

JO - Genetics

JF - Genetics

IS - 1

ER -