Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring

TY - JOUR

T1 - Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice

T2 - effects on liver DNA strand breaks in dams and offspring

AU - Jackson, Petra

AU - Hougaard, Karin Sørig

AU - Boisen, Anne Mette Zenner

AU - Jacobsen, Nicklas Raun

AU - Jensen, Keld Alstrup

AU - Møller, Peter

AU - Brunborg, Gunnar

AU - Gutzkow, Kristine Bjerve

AU - Andersen, Ole

AU - Loft, Steffen

AU - Vogel, Ulla Birgitte

AU - Wallin, Håkan

PY - 2012/8

Y1 - 2012/8

N2 - Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m(3) Printex 90 for 1 h/day on gestation days (GD) 8-18, or by four intratracheal instillations on GD 7, 10, 15 and 18, with total doses of 11, 54 and 268 µg/animal. Dams were monitored until weaning and some offspring until adolescence. Inflammation was assessed in maternal bronchoalveolar lavage (BAL) 3-5 days after exposure, and at weaning. Levels of DNA strand breaks were assessed in maternal BAL cells and liver, and in offspring liver. Persistent lung inflammation was observed in exposed mothers. Inhalation exposure induced more DNA strand breaks in the liver of mothers and their offspring, whereas intratracheal instillation did not. Neither inhalation nor instillation affected gestation and lactation. Maternal inhalation exposure to Printex 90-induced liver DNA damage in the mothers and the in utero exposed offspring.

AB - Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m(3) Printex 90 for 1 h/day on gestation days (GD) 8-18, or by four intratracheal instillations on GD 7, 10, 15 and 18, with total doses of 11, 54 and 268 µg/animal. Dams were monitored until weaning and some offspring until adolescence. Inflammation was assessed in maternal bronchoalveolar lavage (BAL) 3-5 days after exposure, and at weaning. Levels of DNA strand breaks were assessed in maternal BAL cells and liver, and in offspring liver. Persistent lung inflammation was observed in exposed mothers. Inhalation exposure induced more DNA strand breaks in the liver of mothers and their offspring, whereas intratracheal instillation did not. Neither inhalation nor instillation affected gestation and lactation. Maternal inhalation exposure to Printex 90-induced liver DNA damage in the mothers and the in utero exposed offspring.

KW - Administration, Inhalation

KW - Animals

KW - Bronchoalveolar Lavage Fluid

KW - DNA Damage

KW - Drug-Induced Liver Injury

KW - Female

KW - Inhalation Exposure

KW - Lactation

KW - Liver

KW - Male

KW - Maternal Exposure

KW - Mice

KW - Mice, Inbred C57BL

KW - Nanoparticles

KW - Organ Size

KW - Pneumonia

KW - Pregnancy

KW - Soot

U2 - 10.3109/17435390.2011.587902

DO - 10.3109/17435390.2011.587902

M3 - Journal article

C2 - 21649560

SN - 1743-5390

VL - 6

SP - 486

EP - 500

JO - Nanotoxicology

JF - Nanotoxicology

IS - 5

ER -