Progress in developing PNA as a gene-targeted drug

TY - JOUR

T1 - Progress in developing PNA as a gene-targeted drug

AU - Good, L

AU - Nielsen, Peter E.

PY - 1997/8

Y1 - 1997/8

N2 - Peptide nucleic acid (PNA) is a DNA mimic in which the nucleobases are attached to a pseudopeptide backbone. This achiral, uncharged, and rather flexible peptide backbone permits more stable hybridization to DNA and RNA oligomers with uncompromised or even improved sequence selectivity. Additional advantages of PNA are stability against nucleases and proteases and convenient solid phase synthesis. At the RNA level, PNA can be targeted to mRNA to block protein synthesis in an antisense strategy. PNA can also be targeted to the RNA component of ribonucleoproteins (RNPs) to inhibit their enzymatic activities. At the DNA level, the unique ability of PNA to bind DNA by duplex invasion can be used to arrest transcription within a gene sequence or to provide an artificial open complex to promote transcription. This review focuses on recent progress toward the development of PNA as a sequence-targeted drug.

AB - Peptide nucleic acid (PNA) is a DNA mimic in which the nucleobases are attached to a pseudopeptide backbone. This achiral, uncharged, and rather flexible peptide backbone permits more stable hybridization to DNA and RNA oligomers with uncompromised or even improved sequence selectivity. Additional advantages of PNA are stability against nucleases and proteases and convenient solid phase synthesis. At the RNA level, PNA can be targeted to mRNA to block protein synthesis in an antisense strategy. PNA can also be targeted to the RNA component of ribonucleoproteins (RNPs) to inhibit their enzymatic activities. At the DNA level, the unique ability of PNA to bind DNA by duplex invasion can be used to arrest transcription within a gene sequence or to provide an artificial open complex to promote transcription. This review focuses on recent progress toward the development of PNA as a sequence-targeted drug.

KW - Base Sequence

KW - DNA/chemistry

KW - Deoxyribonucleases

KW - Drug Carriers

KW - Endopeptidases

KW - Nucleic Acid Conformation

KW - Nucleic Acids

KW - Peptides/chemical synthesis

KW - RNA/drug effects

KW - Ribosomes/drug effects

KW - Transcription, Genetic/drug effects

U2 - 10.1089/oli.1.1997.7.431

DO - 10.1089/oli.1.1997.7.431

M3 - Review

C2 - 9303195

SN - 2159-3337

VL - 7

SP - 431

EP - 437

JO - Nucleic Acid Therapeutics

JF - Nucleic Acid Therapeutics

IS - 4

ER -