Production of recombinant peanut allergen Ara h 2 using Lactococcus lactis

TY - JOUR

T1 - Production of recombinant peanut allergen Ara h 2 using Lactococcus lactis

AU - Glenting, Jacob

AU - Poulsen, Lars K.

AU - Kato, Kentaro

AU - Madsen, Søren

AU - Frøkiær, Hanne

AU - Wendt, Camilla

AU - Sørensen, Helle W

PY - 2007

Y1 - 2007

N2 - Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may lead to unpredictable efficacy and/or unacceptable levels of side effects. The use of recombinant expression systems for allergen production can alleviate some of these issues. Several allergens have been tested in high-level expression systems and in most cases show immunereactivity comparable to their natural counterparts. The gram positive lactic acid bacterium Lactococcus lactis is an attractive microorganism for use in the production of protein therapeutics. L. lactis is considered food grade, free of endotoxins, and is able to secrete the heterologous product together with few other native proteins. Hypersensitivity to peanut represents a serious allergic problem. Some of the major allergens in peanut have been described. However, for therapeutic usage more information about the individual allergenic components is needed. In this paper we report recombinant production of the Ara h 2 peanut allergen using L. lactis.

AB - Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may lead to unpredictable efficacy and/or unacceptable levels of side effects. The use of recombinant expression systems for allergen production can alleviate some of these issues. Several allergens have been tested in high-level expression systems and in most cases show immunereactivity comparable to their natural counterparts. The gram positive lactic acid bacterium Lactococcus lactis is an attractive microorganism for use in the production of protein therapeutics. L. lactis is considered food grade, free of endotoxins, and is able to secrete the heterologous product together with few other native proteins. Hypersensitivity to peanut represents a serious allergic problem. Some of the major allergens in peanut have been described. However, for therapeutic usage more information about the individual allergenic components is needed. In this paper we report recombinant production of the Ara h 2 peanut allergen using L. lactis.

U2 - 10.1186/1475-2859-6-28

DO - 10.1186/1475-2859-6-28

M3 - Journal article

C2 - 17711578

SN - 1475-2859

VL - 6

JO - Microbial Cell

JF - Microbial Cell

M1 - 28

ER -