Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial

TY - JOUR

T1 - Probiotics and the immunological response to infant vaccinations; a double-blind randomized controlled trial

AU - Sørensen, Camilla Adler

AU - Fuglsang, Eva

AU - Jørgensen, Charlotte Sværke

AU - Laursen, Rikke Pilmann

AU - Larnkjær, Anni

AU - Mølgaard, Christian

AU - Ritz, Christian

AU - Michaelsen, Kim F.

AU - Krogfelt, Karen Angeliki

AU - Frøkiær, Hanne

N1 - CURIS 2018 NEXS 317

PY - 2019/4

Y1 - 2019/4

N2 - Objectives: To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in healthy Danish children, aged 8–14 months, at the time of starting day care. Moreover, the cytokine response to lipopolysaccharide of whole blood was assessed. Methods: A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a 6-month intervention period, and blood samples were drawn at the start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin-induced interleukin-6 (IL-6) and interferon-γ (IFN-γ) production in blood were analysed by Luminex and ELISA. Results: There was no significant difference between the average individual changes from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% versus placebo 238.8% ± 1.1%, p 0.340). The average individual change in IL-6 concentration was significantly lower in the probiotics versus the placebo group (2.9% ± 10.3% versus 33.7% ± 9.0%, p 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% versus –0.2% ± 0.1%, p 0.279). Conclusions: The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.

AB - Objectives: To examine the effect of a combination of probiotics on the antibody response to pneumococcal and pertussis vaccination in healthy Danish children, aged 8–14 months, at the time of starting day care. Moreover, the cytokine response to lipopolysaccharide of whole blood was assessed. Methods: A total of 290 children were randomly allocated to receive a combination of Bifidobacterium animalis ssp. lactis and Lactobacillus rhamnosus GG daily for a 6-month intervention period, and blood samples were drawn at the start and end of the study. Specific antibody response towards Streptococcus pneumoniae serotypes and Bordetella pertussis toxin, as well as endotoxin-induced interleukin-6 (IL-6) and interferon-γ (IFN-γ) production in blood were analysed by Luminex and ELISA. Results: There was no significant difference between the average individual changes from baseline to end of study in antibody concentrations for S. pneumoniae for both the probiotics (340.4% ± 11.2%) and the placebo group (382.9% ± 10.4%) (p 0.525), nor for B. pertussis toxin in the two groups (probiotics 190.1% ± 12.6% versus placebo 238.8% ± 1.1%, p 0.340). The average individual change in IL-6 concentration was significantly lower in the probiotics versus the placebo group (2.9% ± 10.3% versus 33.7% ± 9.0%, p 0.024), whereas there was no difference in IFN-γ concentration (0.0% ± 0.2% versus –0.2% ± 0.1%, p 0.279). Conclusions: The probiotic intervention did not affect the antibody response against S. pneumoniae and B. pertussis toxin in healthy Danish children.

KW - Faculty of Science

KW - Cytokine activation

KW - Pertussis

KW - Placebo-controlled study

KW - Pneumococcus

KW - Probiotics

KW - Vaccination response

U2 - 10.1016/j.cmi.2018.07.031

DO - 10.1016/j.cmi.2018.07.031

M3 - Journal article

C2 - 30099133

SN - 1198-743X

VL - 25

SP - 511.e1-511.e7

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 4

ER -