Peptide nucleic acid.DNA strand displacement loops as artificial transcription promoters

N E Møllegaard; O Buchardt; Peter E. Nielsen

Peptide nucleic acid.DNA strand displacement loops as artificial transcription promoters

N E Møllegaard, O Buchardt, Peter E. Nielsen

Transcription, RNA, and Gene Medicine Program

158 Citationer (Scopus)

Abstract

Homopyrimidine peptide nucleic acids (PNAs) form loop structures when binding to complementary double-stranded DNA by strand displacement, and we now show that RNA polymerase recognizes these and initiates RNA transcription from PNA/double-stranded DNA strand displacement complexes at an efficiency comparable to that of the strong Escherichia coli lacUV5 promoter. Thus PNA targets can be considered as artificial promoters controlled positively by the corresponding PNA as a transcription factor. Our results have implications for the mechanism of action of RNA polymerase and suggest the use of PNA as specific gene activating reagents and drugs.

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Sciences of the United States of America
Vol/bind	91
Udgave nummer	9
Sider (fra-til)	3892-5
Antal sider	4
ISSN	0027-8424
Status	Udgivet - 26 apr. 1994

Citationsformater

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TY - JOUR

T1 - Peptide nucleic acid.DNA strand displacement loops as artificial transcription promoters

AU - Møllegaard, N E

AU - Buchardt, O

AU - Nielsen, Peter E.

PY - 1994/4/26

Y1 - 1994/4/26

N2 - Homopyrimidine peptide nucleic acids (PNAs) form loop structures when binding to complementary double-stranded DNA by strand displacement, and we now show that RNA polymerase recognizes these and initiates RNA transcription from PNA/double-stranded DNA strand displacement complexes at an efficiency comparable to that of the strong Escherichia coli lacUV5 promoter. Thus PNA targets can be considered as artificial promoters controlled positively by the corresponding PNA as a transcription factor. Our results have implications for the mechanism of action of RNA polymerase and suggest the use of PNA as specific gene activating reagents and drugs.

AB - Homopyrimidine peptide nucleic acids (PNAs) form loop structures when binding to complementary double-stranded DNA by strand displacement, and we now show that RNA polymerase recognizes these and initiates RNA transcription from PNA/double-stranded DNA strand displacement complexes at an efficiency comparable to that of the strong Escherichia coli lacUV5 promoter. Thus PNA targets can be considered as artificial promoters controlled positively by the corresponding PNA as a transcription factor. Our results have implications for the mechanism of action of RNA polymerase and suggest the use of PNA as specific gene activating reagents and drugs.

KW - Animals

KW - Cell Nucleus/metabolism

KW - DNA/chemistry

KW - DNA-Directed RNA Polymerases/metabolism

KW - Deoxyribonucleoproteins/chemistry

KW - Escherichia coli/enzymology

KW - In Vitro Techniques

KW - Nucleic Acid Conformation

KW - Oligodeoxyribonucleotides/chemistry

KW - Oligonucleotides, Antisense/chemistry

KW - Peptides/chemistry

KW - Promoter Regions, Genetic

KW - Rats

KW - Transcription, Genetic

M3 - Journal article

C2 - 8171008

SN - 0027-8424

VL - 91

SP - 3892

EP - 3895

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -

Peptide nucleic acid.DNA strand displacement loops as artificial transcription promoters

Abstract

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