TY - JOUR
T1 - PDGF-induced phosphorylation of Tyr28 in the N-terminus of Fyn affects Fyn activation.
AU - Hansen, Klaus
AU - Alonso, G
AU - Courtneidge, S A
AU - Rönnstrand, L
AU - Heldin, C H
N1 - Keywords: 3T3 Cells; Amino Acid Sequence; Animals; DNA Mutational Analysis; Enzyme Activation; Humans; Mice; Molecular Sequence Data; Peptide Mapping; Phosphorylation; Phosphotyrosine; Platelet-Derived Growth Factor; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Receptor Protein-Tyrosine Kinases; Receptor, Platelet-Derived Growth Factor beta; Receptors, Platelet-Derived Growth Factor; Signal Transduction; Tyrosine; src-Family Kinases
PY - 1997
Y1 - 1997
N2 - Binding of platelet-derived growth factor (PDGF) to its receptors leads to the activation of members of the Src family of protein tyrosine kinases. We show here that Fyn, a member of the Src family, is phosphorylated on Tyr28 in the unique N-terminal part of the molecule after interaction with the intracellular domain of the PDGF beta-receptor. Activated Fyn furthermore undergoes autophosphorylation on Tyr30, Tyr39 and Tyr420. When Fyn mutants with Tyr28, Tyr30 or Tyr39 replaced with phenylalanine residues were transfected into NIH3T3 cells a decreased activation after PDGF stimulation was seen, suggesting a functional importance of the N-terminal tyrosine phosphorylation of Fyn.
AB - Binding of platelet-derived growth factor (PDGF) to its receptors leads to the activation of members of the Src family of protein tyrosine kinases. We show here that Fyn, a member of the Src family, is phosphorylated on Tyr28 in the unique N-terminal part of the molecule after interaction with the intracellular domain of the PDGF beta-receptor. Activated Fyn furthermore undergoes autophosphorylation on Tyr30, Tyr39 and Tyr420. When Fyn mutants with Tyr28, Tyr30 or Tyr39 replaced with phenylalanine residues were transfected into NIH3T3 cells a decreased activation after PDGF stimulation was seen, suggesting a functional importance of the N-terminal tyrosine phosphorylation of Fyn.
U2 - 10.1006/bbrc.1997.7743
DO - 10.1006/bbrc.1997.7743
M3 - Journal article
C2 - 9425276
SN - 0006-291X
VL - 241
SP - 355
EP - 362
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -