Pattern of pre-existing IgG subclass responses to a panel of asexual stage malaria antigens reported during the lengthy dry season in Daraweesh, Sudan

TY - JOUR

T1 - Pattern of pre-existing IgG subclass responses to a panel of asexual stage malaria antigens reported during the lengthy dry season in Daraweesh, Sudan

AU - Nasr, A

AU - Iriemenam, N C

AU - Troye-Blomberg, M

AU - Arnot, D

AU - Theander, T G

AU - Berzins, K

AU - Giha, H A

AU - Elghazali, G

N1 - © 2011 The Authors. Scandinavian Journal of Immunology © 2011 Blackwell Publishing Ltd.

PY - 2011/10/1

Y1 - 2011/10/1

N2 - The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses to a panel of malaria antigens, MSP2-3D7, MSP2-FC27, AMA-1 and Pf332-C231. The results showed that the antibody responses were predominantly age dependent, antigen specific, and their lifespan was at least 5-6 month long. Generally, the IgG3 was most abundant IgG subclass, and the most recognized antigen was Pf332-C231. Furthermore, the correlation between the levels of IgG subclasses was strongest between IgG1 and IgG3, which were more predictive to the total IgG levels. Finally, the response pattern of each of the IgG subclasses to the different test antigens that were spanning the dry season and the correlation between these responses were described in details for the first time.

AB - The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses to a panel of malaria antigens, MSP2-3D7, MSP2-FC27, AMA-1 and Pf332-C231. The results showed that the antibody responses were predominantly age dependent, antigen specific, and their lifespan was at least 5-6 month long. Generally, the IgG3 was most abundant IgG subclass, and the most recognized antigen was Pf332-C231. Furthermore, the correlation between the levels of IgG subclasses was strongest between IgG1 and IgG3, which were more predictive to the total IgG levels. Finally, the response pattern of each of the IgG subclasses to the different test antigens that were spanning the dry season and the correlation between these responses were described in details for the first time.

KW - Adolescent

KW - Adult

KW - Aged

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Child

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Immunoglobulin G

KW - Malaria

KW - Male

KW - Membrane Proteins

KW - Middle Aged

KW - Plasmodium falciparum

KW - Polymerase Chain Reaction

KW - Protozoan Proteins

KW - Seasons

KW - Sudan

U2 - 10.1111/j.1365-3083.2011.02585.x

DO - 10.1111/j.1365-3083.2011.02585.x

M3 - Journal article

C2 - 21645028

SN - 0300-9475

VL - 74

SP - 390

EP - 396

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

IS - 4

ER -