Novel isolates of Streptococcus thermophilus bacteriophages from group 5093 identified with an improved multiplex PCR typing method

TY - JOUR

T1 - Novel isolates of Streptococcus thermophilus bacteriophages from group 5093 identified with an improved multiplex PCR typing method

AU - Szymczak, Paula

AU - Vogensen, Finn Kvist

AU - Janzen, Thomas

PY - 2019

Y1 - 2019

N2 - Bacteriophages are a persistent problem in dairy plants. Streptococcus thermophilus is a component of thermophilic starter cultures used for cheese and yoghurt production. In this study, a multiplex PCR method was adapted to identify, in a single reaction, all known S. thermophilus phage groups. The method was compared with the previously published multiplex PCR typing method and showed higher specificity for classifying purified phage isolates. Three new representatives of phage group 5093, which were discovered with the developed PCR method, were genome sequenced and the genomes were compared with those available in GenBank. A validation of the two typing methods performed with whey revealed limitations of multiplex PCR assays when applied for the detection of phages in dairy samples. This study established an improved multiplex PCR method for classifying purified S. thermophilus phages, and expanded the genetic information of group 5093, both important steps to control phage infections in dairies.

AB - Bacteriophages are a persistent problem in dairy plants. Streptococcus thermophilus is a component of thermophilic starter cultures used for cheese and yoghurt production. In this study, a multiplex PCR method was adapted to identify, in a single reaction, all known S. thermophilus phage groups. The method was compared with the previously published multiplex PCR typing method and showed higher specificity for classifying purified phage isolates. Three new representatives of phage group 5093, which were discovered with the developed PCR method, were genome sequenced and the genomes were compared with those available in GenBank. A validation of the two typing methods performed with whey revealed limitations of multiplex PCR assays when applied for the detection of phages in dairy samples. This study established an improved multiplex PCR method for classifying purified S. thermophilus phages, and expanded the genetic information of group 5093, both important steps to control phage infections in dairies.

U2 - 10.1016/j.idairyj.2018.12.001

DO - 10.1016/j.idairyj.2018.12.001

M3 - Journal article

AN - SCOPUS:85060092309

SN - 0958-6946

VL - 91

SP - 18

EP - 24

JO - International Dairy Journal

JF - International Dairy Journal

ER -