Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

L. E. Amoah; S. V. Nuvor; E. K. Obboh; F. K. Acquah; K. Asare; S. K. Singh; J. N. Boampong; M. Theisen; K. C. Williamson

doi:10.1186/s13071-017-2338-7

Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

L. E. Amoah^*, S. V. Nuvor, E. K. Obboh, F. K. Acquah, K. Asare, S. K. Singh, J. N. Boampong, M. Theisen, K. C. Williamson

^*Corresponding author af dette arbejde

16 Citationer (Scopus)

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Abstract

Background: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7-1035300) and MSP3 (PF3D7-1035400) antigens are associated with decreased parasite load.

Methods: Dried filter paper blood blots were obtained from children and adults diagnosed with uncomplicated P. falciparum malaria. Microscopy was used to estimate P. falciparum parasite density and polymerase chain reaction (PCR) amplification of the polymorphic regions of msp1 (PF3D7-0930300) and msp2 (PF3D7-0206800) was used for parasite genotyping and MOI determination. ELISA was used to measure the serum IgG concentration of R0 fragment of GLURP (GLURP(R0)) and MSP3 antibodies.

Results: All 115 samples were positive for P. falciparum by PCR using either the msp1 or msp2 genotyping primer sets. The most prevalent msp1 and msp2 alleles were KI and 3D7, respectively. The geometric mean (GM) for MOI determined by both msp1 and msp2 genotyping was 1.3 for the entire population and was generally higher in children than in adults. Seropositivity was estimated at 67 and 63% for GLURP(R0) and MSP3 antibodies, respectively, and antibody titers were negatively correlated with parasite density.

Conclusions: The negative correlation between naturally occurring GLURP(R0) and MSP3 antibody levels and parasite density observed in this study suggest that augmenting the antibody response with the GMZ2 vaccine could enhance protection in the Central Region of Ghana.

Originalsprog	Engelsk
Artikelnummer	395
Tidsskrift	Parasites and Vectors
Vol/bind	10
Udgave nummer	1
Antal sider	9
ISSN	1756-3305
DOI	https://doi.org/10.1186/s13071-017-2338-7
Status	Udgivet - 2017

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10.1186/s13071-017-2338-7Licens: CC BY

Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of GhanaForlagets udgivne version, 850 KBLicens: CC BY

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Amoah, L. E., Nuvor, S. V., Obboh, E. K., Acquah, F. K., Asare, K., Singh, S. K., Boampong, J. N., Theisen, M., & Williamson, K. C. (2017). Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana. Parasites and Vectors, 10(1), [395]. https://doi.org/10.1186/s13071-017-2338-7

Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana. / Amoah, L. E.; Nuvor, S. V.; Obboh, E. K. et al.

I: Parasites and Vectors, Bind 10, Nr. 1, 395, 2017.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Amoah, LE, Nuvor, SV, Obboh, EK, Acquah, FK, Asare, K, Singh, SK, Boampong, JN, Theisen, M & Williamson, KC 2017, 'Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana', Parasites and Vectors, bind 10, nr. 1, 395. https://doi.org/10.1186/s13071-017-2338-7

@article{74b305e984e445d29ab364fdfef045e0,

title = "Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana",

abstract = "Background: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7-1035300) and MSP3 (PF3D7-1035400) antigens are associated with decreased parasite load. Methods: Dried filter paper blood blots were obtained from children and adults diagnosed with uncomplicated P. falciparum malaria. Microscopy was used to estimate P. falciparum parasite density and polymerase chain reaction (PCR) amplification of the polymorphic regions of msp1 (PF3D7-0930300) and msp2 (PF3D7-0206800) was used for parasite genotyping and MOI determination. ELISA was used to measure the serum IgG concentration of R0 fragment of GLURP (GLURP(R0)) and MSP3 antibodies. Results: All 115 samples were positive for P. falciparum by PCR using either the msp1 or msp2 genotyping primer sets. The most prevalent msp1 and msp2 alleles were KI and 3D7, respectively. The geometric mean (GM) for MOI determined by both msp1 and msp2 genotyping was 1.3 for the entire population and was generally higher in children than in adults. Seropositivity was estimated at 67 and 63% for GLURP(R0) and MSP3 antibodies, respectively, and antibody titers were negatively correlated with parasite density. Conclusions: The negative correlation between naturally occurring GLURP(R0) and MSP3 antibody levels and parasite density observed in this study suggest that augmenting the antibody response with the GMZ2 vaccine could enhance protection in the Central Region of Ghana.",

keywords = "Genetic diversity, GLURP, msp1, msp2, MSP3, Multiplicity of infection",

author = "Amoah, {L. E.} and Nuvor, {S. V.} and Obboh, {E. K.} and Acquah, {F. K.} and K. Asare and Singh, {S. K.} and Boampong, {J. N.} and M. Theisen and Williamson, {K. C.}",

year = "2017",

doi = "10.1186/s13071-017-2338-7",

language = "English",

volume = "10",

journal = "Parasites & Vectors",

issn = "1756-3305",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

AU - Amoah, L. E.

AU - Nuvor, S. V.

AU - Obboh, E. K.

AU - Acquah, F. K.

AU - Asare, K.

AU - Singh, S. K.

AU - Boampong, J. N.

AU - Theisen, M.

AU - Williamson, K. C.

PY - 2017

Y1 - 2017

N2 - Background: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7-1035300) and MSP3 (PF3D7-1035400) antigens are associated with decreased parasite load. Methods: Dried filter paper blood blots were obtained from children and adults diagnosed with uncomplicated P. falciparum malaria. Microscopy was used to estimate P. falciparum parasite density and polymerase chain reaction (PCR) amplification of the polymorphic regions of msp1 (PF3D7-0930300) and msp2 (PF3D7-0206800) was used for parasite genotyping and MOI determination. ELISA was used to measure the serum IgG concentration of R0 fragment of GLURP (GLURP(R0)) and MSP3 antibodies. Results: All 115 samples were positive for P. falciparum by PCR using either the msp1 or msp2 genotyping primer sets. The most prevalent msp1 and msp2 alleles were KI and 3D7, respectively. The geometric mean (GM) for MOI determined by both msp1 and msp2 genotyping was 1.3 for the entire population and was generally higher in children than in adults. Seropositivity was estimated at 67 and 63% for GLURP(R0) and MSP3 antibodies, respectively, and antibody titers were negatively correlated with parasite density. Conclusions: The negative correlation between naturally occurring GLURP(R0) and MSP3 antibody levels and parasite density observed in this study suggest that augmenting the antibody response with the GMZ2 vaccine could enhance protection in the Central Region of Ghana.

AB - Background: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7-1035300) and MSP3 (PF3D7-1035400) antigens are associated with decreased parasite load. Methods: Dried filter paper blood blots were obtained from children and adults diagnosed with uncomplicated P. falciparum malaria. Microscopy was used to estimate P. falciparum parasite density and polymerase chain reaction (PCR) amplification of the polymorphic regions of msp1 (PF3D7-0930300) and msp2 (PF3D7-0206800) was used for parasite genotyping and MOI determination. ELISA was used to measure the serum IgG concentration of R0 fragment of GLURP (GLURP(R0)) and MSP3 antibodies. Results: All 115 samples were positive for P. falciparum by PCR using either the msp1 or msp2 genotyping primer sets. The most prevalent msp1 and msp2 alleles were KI and 3D7, respectively. The geometric mean (GM) for MOI determined by both msp1 and msp2 genotyping was 1.3 for the entire population and was generally higher in children than in adults. Seropositivity was estimated at 67 and 63% for GLURP(R0) and MSP3 antibodies, respectively, and antibody titers were negatively correlated with parasite density. Conclusions: The negative correlation between naturally occurring GLURP(R0) and MSP3 antibody levels and parasite density observed in this study suggest that augmenting the antibody response with the GMZ2 vaccine could enhance protection in the Central Region of Ghana.

KW - Genetic diversity

KW - GLURP

KW - msp1

KW - msp2

KW - MSP3

KW - Multiplicity of infection

U2 - 10.1186/s13071-017-2338-7

DO - 10.1186/s13071-017-2338-7

M3 - Journal article

C2 - 28835262

AN - SCOPUS:85028337296

SN - 1756-3305

VL - 10

JO - Parasites & Vectors

JF - Parasites & Vectors

IS - 1

M1 - 395

ER -

Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

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