Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

TY - JOUR

T1 - Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

AU - Pinela da Silva, Sonia Cristina

AU - Altmannova, Veronika

AU - Luke-Glaser, Sarah

AU - Henriksen, Peter

AU - Gallina, Irene

AU - Yang, Xuejiao

AU - Choudhary, Chuna Ram

AU - Luke, Brian

AU - Krejci, Lumir

AU - Lisby, Michael

N1 - © 2016 Silva et al.; Published by Cold Spring Harbor Laboratory Press.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize atDNAdamage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

AB - Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize atDNAdamage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

U2 - 10.1101/gad.276204.115

DO - 10.1101/gad.276204.115

M3 - Journal article

C2 - 26966248

SN - 0890-9369

VL - 30

SP - 700

EP - 717

JO - Genes & Development

JF - Genes & Development

IS - 6

ER -